preQ1-biotin

Tocris Bioscience | Catalog # 7804

PreQ1 with biotin handle for RNA labeling and purification
Tocris Bioscience

Key Product Details

Description

PreQ1 with biotin handle for RNA labeling and purification
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Product Description

preQ1-biotin is a modified analog of the natural substrate prequeosine1 (preQ1) and is used for RNA-TAG (transglycosylation at guanosine) and DNA-TAG. preQ1-biotin enables affinity tagging and pull-down of specific RNAs that have been modified selectively by E. coli tRNA guanine transglycosylase (TGT). preQ1-biotin is incorporated site-specifically and covalently into RNAs containing a short harpin nucleotide recognition motif. The guanine in a UGU recognition element is exchanged with the preQ1-biotin substrate.

Plasmids for expression of the E.coli TGT enzyme (#138201) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end (#138209) are available from Addgene.

Scientific Data Images for preQ1-biotin

Schematic representation of preQ1-bitoin insertion into DNA.

E. coli TGT irreversibly exchanges a specific guanine in a DNA hairpin with preQ1-biotin.

Schematic representation of preQ1-bitoin insertion into DNA.
Urea-PAGE gel shift analysis of preQ1-biotin insertion into a DNA

E. coli TGT irreversibly exchanges a specific guanine in a DNA hairpin with preQ1-biotin.

Urea-PAGE gel shift analysis of preQ1-biotin insertion. Modification is evidenced by an upward shift of the product band due to molecular weight increase. Lane 1 is unmodified DNA; lane 2 is preQ1-biotin modified DNA.

Licensing Information

Sold under license from The Regents of the University of California.

Product Specifications for preQ1-biotin

Molecular Weight

504.65

Formula

C23H36N8O3S

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

N-(6-(((2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl)amino)hexyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide

CAS Number

2971850-25-4

InChI Key

APUALFAJXKNNBF-BXWFABGCSA-N

SMILES

O=C1C2=C(NC=C2CNCCCCCCNC(CCCC[C@H]3[C@]4([H])[C@](CS3)(NC(N4)=O)[H])=O)N=C(N)N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 10.09 20
Ethanol 2.52 5

Preparing Stock Solutions for preQ1-biotin

The following data is based on the product molecular weight 504.65.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 9.91 mL 49.54 mL 99.08 mL
1 mM 1.98 mL 9.91 mL 19.82 mL
2 mM 0.99 mL 4.95 mL 9.91 mL
10 mM 0.20 mL 0.99 mL 1.98 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for preQ1-biotin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for preQ1-biotin

For research use only

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