PRKCDBP Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-47323

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VHAVTVVTLLEKLASMLETLRERQGGLARRQGGLAGSVRRIQSGLGALSRSHDTTSNTLAQLLAKAERVSSHANAAQE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRKCDBP Antibody - BSA Free

Western Blot: PRKCDBP Antibody [NBP2-47323]

Western Blot: PRKCDBP Antibody [NBP2-47323]

Western Blot: PRKCDBP Antibody [NBP2-47323] - Analysis in control (vector only transfected HEK293T lysate) and PRKCDBP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PRKCDBP Antibody [NBP2-47323]

Immunohistochemistry-Paraffin: PRKCDBP Antibody [NBP2-47323]

Immunohistochemistry-Paraffin: PRKCDBP Antibody [NBP2-47323] - Staining of human smooth muscle shows strong membranous positivity in smooth muscle cells.
Western Blot: PRKCDBP Antibody [NBP2-47323]

Western Blot: PRKCDBP Antibody [NBP2-47323]

Western Blot: PRKCDBP Antibody [NBP2-47323] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line U-87 MG.

Applications for PRKCDBP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRKCDBP

PRKCDBP, also known as Protein kinase C delta-binding protein, is 261 amino acids in length and approximately 27kDa. PRKCDBP is highly expressed in epithelial cells, and is down regulated in lung cancer cell lines and breast cancer cell lines, thus suggesting the possibility of tumor suppressor function. PRKCDBP binds and is phosphorylated by protein kinase C, delta. Current research surrounding PRKCDBP shows a possible connection with ovarian cancer, gastric cancer and lung cancer. PRKCDBP has also been shown to interact with Caveolin 1, Caveolin 3, cMyc, SDPR, and EPS15L1.

Alternate Names

cavin-3, HSRBC, MGC20400, protein kinase C, delta binding protein, sdr-related gene product that binds to c-kinase, Serum deprivation response factor-related gene product that binds to C-kinase, SRBCprotein kinase C delta-binding protein

Gene Symbol

CAVIN3

Additional PRKCDBP Products

Product Documents for PRKCDBP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PRKCDBP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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