Q: We have a customer who is looking for an antibody that detect endogenous PRMT8 and found Cat.No.NBP1-55401 and NBP1-81702 (anti-PRMT8). The customer would like some further information on how you verified that the antibody indeed detects PRMT8,and only PRMT8. Kat.Nr. NBP1-55401 is generated against the C-term of PRMT8, and when looking at the allignment of PRMT8 and PRMT1, the C-terminus shows high homology (please see attached). Do you have any further information what this antibody detects or has the antibody never been tested in anything else than the transfected cell lysate? (PRMT1) For Kat.Nr. NBP1-81702, do you have any data showing endogenous protein in Western Blot? Will Cat.No. NBP1-81702 be guaranteed to detect endogenous protein in Western Blot, since it does detect endogenous protein in brain tissue sections in IHC?

Question

Q: We have a customer who is looking for an antibody that detect endogenous PRMT8 and found Cat.No.NBP1-55401 and NBP1-81702 (anti-PRMT8).  The customer would like some further information on how you verified that the antibody indeed detects PRMT8,and only PRMT8. Kat.Nr. NBP1-55401 is generated against the C-term of PRMT8, and when looking at the allignment of PRMT8 and PRMT1, the C-terminus shows high homology (please see attached). Do you have any further information what this antibody detects or has the antibody never been tested in anything else than the transfected cell lysate?  (PRMT1) For Kat.Nr. NBP1-81702, do you have any data showing endogenous protein in Western Blot? Will Cat.No. NBP1-81702 be guaranteed to detect endogenous protein in Western Blot, since it does detect endogenous protein in brain tissue sections in IHC?

Accepted Answer

A: Our PRMT8 antibody with catalogue number NBP1-55401 was raised to an immunogen sequence derived from the C-terminus of human PRMT8 which, as you rightly point out, shares 78% sequence homology with PRMT1. Unfortunately we have not tested the cross-reactivity of this antibody with PRMT1 and so I am unable to confirm whether or not it recognises PRMT1. The only testing data we currently have is that which is shown on our website, the Western blot image using lysate from transfected 293T cells. NBP1-81702 is covered by our guarantee for detection of PRMT8 in human and mouse samples by Western blotting and IHC with paraffin-embedded samples. Our testing data was again derived using an over-expression lysate and we do not have an image showing detection of the endogenous protein by Western blotting. However, as you know, we stand proudly behind our guarantee that our antibodies will work in the species and applications listed on our website and on our product datasheets.

PRMT8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-55401

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