Q: Could you please tell us whether there are the components containing recombinant protein in this your Mouse proANP ELISA Kit (Colorimetric), Cat#NBP2-68143? If yes, Could you tell us the expression systems of the recombinant protein? Ex. E.coli, Yeast, HEK293 cells, CHO cells and insect cells etc.

A: Mouse proANP ELISA Kit (Colorimetric) cat# NBP2-68143 contains a standard which is a recombinant mouse pro-ANP fragment protein expressed by E.coli.

Mouse proANP ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-68143

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse Pro-ANP in samples. No significant cross-reactivity or interference between Mouse Pro-ANP and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.72%

Inter-Assay Precision (Precision between assays) CV% 4.62%

Recovery for Mouse proANP ELISA Kit (Colorimetric)

Recovery

84-105%

Linearity

Scientific Data Images for Mouse proANP ELISA Kit (Colorimetric)

ELISA: Mouse proANP ELISA Kit (Colorimetric) [NBP2-68143] -

ELISA: Mouse proANP ELISA Kit (Colorimetric) [NBP2-68143] - Standard Curve Reference

Kit Contents for Mouse proANP ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: proANP

The protein encoded by the proANP gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. (provided by RefSeq)

Alternate Names

ANF, ANPatriopeptin, ATFB6, atrial natriuretic factor, Atrial natriuretic peptide, CDD-ANF, natriuretic peptide A, natriuretic peptide precursor A, PNDcardionatrin, Prepronatriodilatin

Gene Symbol

NPPA

Additional proANP Products

Product Documents for Mouse proANP ELISA Kit (Colorimetric)

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Download Manual - Mouse proANP ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mouse proANP ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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