ProDots Recombinant Human G-CSF Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD214-10
PRD214-50
PRD214-01
ProDots Recombinant Human G-CSF Protein Bioactivity
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ProDots Recombinant Human G-CSF Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Features and Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The ED50 for this effect is 10-60 pg/mL.
Source
E. coli-derived human G-CSF protein
Thr31 - Pro204, with an -N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
18.8 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

PRD214

Reconstitution For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot® directly into the cell culture medium for immediate use.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
 • 6 months from date of receipt at room temperature. 
 • 12 months from date of receipt at 2-8 °C as supplied. 
 • 1 month at 2-8 °C under sterile conditions after reconstitution. 
 • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

ProDots®Recombinant Human G-CSF stimulated cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50for this effect is 10-60 pg/mL.

SDS-PAGE View Larger

1 μg/lane of ProDots®Recombinant Human G-CSF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at approximately 18 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: G-CSF

G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.

Long Name
Granulocyte Colony Stimulating Factor
Entrez Gene IDs
1440 (Human); 12985 (Mouse)
Alternate Names
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; Pluripoietin

FAQs

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Reconstitution Buffers

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