ProDots Recombinant Human G-CSF Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD214-10
PRD214-50
PRD214-01
ProDots Recombinant Human G-CSF Protein Bioactivity
2 Images
Product Details
FAQs
Supplemental Products
Reviews

ProDots Recombinant Human G-CSF Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium


Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The ED50 for this effect is 10-60 pg/mL.
Source
E. coli-derived human G-CSF protein
Thr31 - Pro204, with an -N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
18.8 kDa

Product Datasheets

PRD214

Reconstitution For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot® directly into the cell culture medium for immediate use.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
 • 6 months from date of receipt at room temperature. 
 • 12 months from date of receipt at 2-8 °C as supplied. 
 • 1 month at 2-8 °C under sterile conditions after reconstitution. 
 • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

Bioactivity ProDots Recombinant Human G-CSF Protein Bioactivity View Larger

ProDots® Recombinant Human G-CSF stimulated cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is
10-60 pg/mL.

SDS-PAGE ProDots Recombinant Human G-CSF Protein SDS-PAGE View Larger

1 μg/lane of ProDots® Recombinant Human G-CSF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at approximately 18 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: G-CSF

G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.

Long Name
Granulocyte Colony Stimulating Factor
Entrez Gene IDs
1440 (Human); 12985 (Mouse)
Alternate Names
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for ProDots Recombinant Human G-CSF Protein

There are currently no reviews for this product. Be the first to review ProDots Recombinant Human G-CSF Protein and earn rewards!

Have you used ProDots Recombinant Human G-CSF Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review