G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.
ProDots Recombinant Human G-CSF Protein
R&D Systems | Catalog # PRD214
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD214 has been discontinued.
View all G-CSF products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
E. coli
Accession Number
Applications
Bioactivity
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Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
E. coli-derived human G-CSF protein
Thr31 - Pro204, with an -N-terminal Met
Thr31 - Pro204, with an -N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
18.8 kDa
Activity
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116.
The ED50 for this effect is 10-60 pg/mL.
The ED50 for this effect is 10-60 pg/mL.
Scientific Data Images for ProDots Recombinant Human G-CSF Protein
ProDots Recombinant Human G-CSF Protein Bioactivity
ProDots®Recombinant Human G-CSF stimulated cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is 10-60 pg/mL.
ProDots Recombinant Human G-CSF Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human G-CSF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at approximately 18 kDa.Formulation, Preparation, and Storage
PRD214
| Reconstitution | For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot® directly into the cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: G-CSF
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
UniProt
Additional G-CSF Products
Product Documents for ProDots Recombinant Human G-CSF Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Human G-CSF Protein
726879.0
For research use only
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