ProDots Recombinant Human KGF/FGF-7 Protein PRD251-10: R&D Systems

ProDots Recombinant Human KGF/FGF-7 Protein

Supplied as ready-to-use 5 ug protein balls
  

Pre-aliquoted, lyophilized protein dots for use in cell culture medium


Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?

ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.


What are ProDots® Proteins?

ProDots® proteins are R&D Systems industry-leading quality, highly bioactive proteins packaged into easy-to-use lyophilized “dots”. Designed to be stable at 4 ºC and to dissolve instantly, ProDots® proteins can be rolled into cell culture media to quickly produce media with a desired working protein concentration.


  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is typically 6-60 ng/mL.
  • Source
    E. coli-derived Cys32 - Thr194, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    19 kDa
  • SDS-PAGE


PRD251
 
Reconstitution For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
 • 6 months from date of receipt at room temperature. 
 • 12 months from date of receipt at 2-8 °C as supplied. 
 • 1 month at 2-8 °C under sterile conditions after reconstitution. 
 • 3 months at -20 to -80 °C under sterile conditions after reconstitution.
Data Images
ProDots® Recombinant Human KGF/FGF-7 stimulated cell proliferation of the 4MBr-5 rhesus monkey epithelial cell line. The ED50 for this effect is typically
6-60 ng/mL.

1 μg/lane of ProDots® Recombinant Human KGF/FGF-7 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 19 kDa.

Background: KGF/FGF-7
KGF (keratinocyte growth factor), also known as FGF-7, is induced in mesenchymal cells by inflammation or tissue damage. It functions as a paracrine factor during wound healing by inducing the proliferation of nearby epithelial cells. KGF plays a role in kidney morphogenesis, hair follicle development, and keratinocyte differentiation. It signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (IIIb)/KGF R.
  • Long Name:
    Keratinocyte Growth Factor
  • Entrez Gene IDs:
    2252 (Human); 403915 (Canine)
  • Alternate Names:
    FGF7; FGF-7; fibroblast growth factor 7HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor)
Related Research Areas

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