KGF (keratinocyte growth factor), also known as FGF-7, is induced in mesenchymal cells by inflammation or tissue damage. It functions as a paracrine factor during wound healing by inducing the proliferation of nearby epithelial cells. KGF plays a role in kidney morphogenesis, hair follicle development, and keratinocyte differentiation. It signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (IIIb)/KGF R.
ProDots Recombinant Human KGF/FGF-7 Protein
R&D Systems | Catalog # PRD251
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD251 has been discontinued.
View all KGF/FGF-7 products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
E. coli
Accession Number
Applications
Bioactivity, SDS-PAGE
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Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
E. coli-derived human KGF/FGF-7 protein
Cys32 - Thr194, with an N-terminal Met
Cys32 - Thr194, with an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
19 kDa
SDS-PAGE
Activity
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802.
The ED50 for this effect is 6-60 ng/mL.
None
The ED50 for this effect is 6-60 ng/mL.
None
Scientific Data Images for ProDots Recombinant Human KGF/FGF-7 Protein
ProDots Recombinant Human KGF/FGF-7 Protein Bioactivity
ProDots®Recombinant Human KGF/FGF-7 stimulated cell proliferation of the 4MBr-5 rhesus monkey epithelial cell line. The ED50 for this effect is 6-60 ng/mL.
ProDots Recombinant Human KGF/FGF-7 Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human KGF/FGF-7 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 19 kDa.Formulation, Preparation, and Storage
PRD251
| Reconstitution | For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: KGF/FGF-7
Long Name
Keratinocyte Growth Factor
Alternate Names
FGF-7, FGF7, HBGF-7, HBGF7
Gene Symbol
FGF7
UniProt
Additional KGF/FGF-7 Products
Product Documents for ProDots Recombinant Human KGF/FGF-7 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Human KGF/FGF-7 Protein
726883.0
For research use only
Related Research Areas
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Associated Pathways
