ProDots Recombinant Human/Mouse/Rat Activin A Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD338-10
PRD338-50
PRD338-01
ProDots Recombinant Human/Mouse/Rat Activin A Protein Bioactivity
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Product Details
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ProDots Recombinant Human/Mouse/Rat Activin A Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium


Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2-1.2 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived Activin A protein
Gly311-Ser426
Accession #
N-terminal Sequence
Analysis
Gly311
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13 kDa (monomer)
SDS-PAGE
14 kDa, reducing conditions, 24 kDa, non-reducing conditions

Product Datasheets

PRD338

Reconstitution For a stock solution, reconstitute at 100 μg/mL in 4 mM HCl, or simply roll ProDot® directly into cell culture medium for immediate use.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied.
• 12 months from date of receipt at 2-8 °C as supplied.
• 1 month at 2-8 °C under sterile conditions after reconstitution.
• 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

Bioactivity ProDots Recombinant Human/Mouse/Rat Activin A Protein Bioactivity View Larger

ProDots® Recombinant Human/Mouse/Rat Activin A induces hemoglobin expression in K562 chronic myelogenous leukemia cells. The ED50 is 0.2-1.2 ng/mL.

SDS-PAGE ProDots Recombinant Human/Mouse/Rat Activin A Protein SDS-PAGE View Larger

1µg/lane of ProDots® Recombinant Human/Mouse/Rat Activin A was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing single bands at 14 kDa and 24 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Activin A

Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Activin proteins have a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and roles in reproductive physiology. Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction.

Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned to date. The activin/inhibin nomenclature reflects the subunit composition of the proteins: Activin A (beta A - beta A), Activin B (beta B - beta B), Activin AB (beta A - beta B), Inhibin A (alpha - beta A) and Inhibin B (alpha - beta B).

Entrez Gene IDs
3624 (Human); 16323 (Mouse)
Alternate Names
Activin A; INHBA

FAQs

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ProDots Recombinant Human/Mouse/Rat Activin A Protein
By Anonymous on 05/17/2016

Very easy to use. A go to product.