Human Prostate Tissue Lysate (Adult Normal)
Novus Biologicals | Catalog # NBP2-47063
Key Product Details
Species
Product Summary for Human Prostate Tissue Lysate (Adult Normal)
Normal Tissue Lysate
Diagnosis: Normal prostateSex: Male
Age: 32
Grade: N/A
Stage: N/A
Normal Tissue Pathology Data
Location: Prostate
Gross findings: No data available
Microscopic Findings
No data available
Preparation Method
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na3VO4
Product Specifications
Application Notes
For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.
Type
Life Stage
Tissue
Tissue Condition
Formulation, Preparation, and Storage
Concentration
Shipping
Storage
Product Documents for Human Prostate Tissue Lysate (Adult Normal)
Certificate of Analysis
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Product Specific Notices for Human Prostate Tissue Lysate (Adult Normal)
The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.
This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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