Protease Inhibitor Cocktail I

Tocris Bioscience | Catalog # 5500

Protease inhibitor cocktail for mammalian cells
Tocris Bioscience

Key Product Details

Description

Protease inhibitor cocktail for mammalian cells
View all Biochemicals and Molecular Biology Compounds »

Product Description

Protease Inhibitor Cocktail I is a blend of protease inhibitors optimized for mammalian cells. Contains the following inhibitors: AEBSF HCl (100 mM), Aprotinin (80 μM), Bestatin (5 mM), E-64 (1.5 mM), Leupeptin (2 mM) and Pepstatin (1 mM). Cocktail is provided as 1 ml of 100X concentrate in DMSO.

Product Specifications for Protease Inhibitor Cocktail I

Storage

Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solubility Soluble in DMSO (supplied pre-dissolved in 1ml DMSO)

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 14 publications citing the usage of this product.

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Product Documents for Protease Inhibitor Cocktail I

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Protease Inhibitor Cocktail I

For research use only

Citations for Protease Inhibitor Cocktail I

Customer Reviews for Protease Inhibitor Cocktail I (4)

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  • Protease Inhibitor Cocktail I
    Name: Chi Ngo
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: AGS, MNK-1, 45, HepG2
    Verified Customer | Posted 09/06/2025
    Protease Inhibitor Cocktail I was added to cell lysis buffer to inhibit protease activity while lysing the cells.
  • Protein extraction HCT116
    Name: Gabriele Strusi
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HCT116
    Verified Customer | Posted 04/11/2025
    Protease inhibitor 100x added to Lysis Buffer 16 for Pluripotent stem cell markers proteome profiler.
    Protease Inhibitor Cocktail I 5500
  • Protein extraction from cancer-adipocytes co-culture
    Name: Caterina Suelzu
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: SVF primary cells
    Verified Customer | Posted 04/11/2025
    Used for protein extraction from cancer-adipocytes co-culture. 100X dilution
    Protease Inhibitor Cocktail I 5500
  • Protein extraction addition
    Name: Murali K Yanda
    Species: protein lysis buffer
    Assay Type: In Vitro
    Cell Line/Tissue: 293
    Verified Customer | Posted 09/27/2024
    We did WB with or without this inhibitor. We can see on the right lane, which is when we add inhibitor, showed protection of protein degradation.
    100X, one to one hundred dilute
    Protease Inhibitor Cocktail I 5500

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