Recombinant Protein A/G/L Cys (N-Term) Protein

Name: Recombinant Protein A/G/L Cys (N-Term) Protein
Brand: Novus Biologicals
SKU: NBP2-34961
Price: 113 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-34961

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Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

Cys (N-Term)

Applications

Bioactivity, SDS-PAGE

View all Protein A/G/L Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 806 amino acids corresponding to Protein A/G/L with a Cys on the N-terminusSource: E. coli

Amino Acid Sequence: CNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE GSGSGTYKLI LNGKTLKGET TTEAVDAATA EKVFKQYAND NGVDGEWTYD DATKTFTVTE KPEVIDASEL TPAVTTYKLV INGKTLKGET TTKAVDAETA EKAFKQYAND NGVDGVWTYD DATKTFTVTE EPRARPGSGS GKEETPETPE TDSEEEVTIK ANLIFANGST QTAEFKGTFE KATSEAYAYA DTLKKDNGEY TVDVADKGYT LNIKFAGKEK TPEEPKEEVT IKANLIYADG KTQTAEFKGT FEEATAEAYR YADALKKDNG EYTVDVADKG YTLNIKFAGK EKTPEEPKEE VTIKANLIYA DGKTQTAEFK GTFEEATAEA YRYADLLAKE NGKYTVDVAD KGYTLNIKFA GKEKTPEEPK EEVTIKANLI YADGKTQTAE FKGTFAEATA EAYRYADLLA KENGKYTADL EDGGYTINIR FAGKKVDEKP EEKEQVTIKE NIYFEDGTVQ TATFKGTFAE ATAEAYRYAD LLSKEHGKYT ADLEDGGYTI NIRFAG

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Protein A/G/L as determined by LAL method.

Predicted Molecular Mass

89.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Protein A/G/L is a genetically engineered protein containing 5 IgG-binding regions of protein A, 2 of protein G and 5 of Protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G/L to ensure the maximum specific IgG binding. The recombinant Protein A/G/L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A/G/L binds to human, mouse, rat, cow, goat, sheep, rabbit, guinea pig, pig, dog and cat IgG.

Specificity

Protein A/G/L binds to human, mouse, rat cow, goat, sheep, rabbit, guinea pig, pig, dog and cat IgG.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-34961

Formulation	Lyophilized from additive free solution.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Dissolve in distilled water or saline.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Protein A/G/L

Protein A/G/L is a genetically engineered protein that combines the IgG binding profiles of all Protein A, Protein G and Protein L. It is a gene fusion product. Recombinant fusion protein A/G/L contains five Ig-binding regions of protein L, five IgG binding domains from Protein A, and two Ig-binding region of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding.

Additional Protein A/G/L Products

Product Documents for Recombinant Protein A/G/L Cys (N-Term) Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Protein A/G/L Cys (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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