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Key Product Details
Accession Number
Conjugate
Applications
Product Specifications
Description
Store SEPHAROSE PROTEIN A at 4C prior to opening. DO NOT FREEZE.
Application Notes
Protein / Peptide Type
Scientific Data Images for Protein A Native Protein
Immunoprecipitation: Protein A Native Protein [NBP1-97288]
Immunoprecipitation: Protein A Native Protein [NBP1-97288] - Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding.Formulation, Preparation, and Storage
NBP1-97288
| Preparation Method | Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose(TM) Protein A can be used for immunoprecipitation and purification of monoclonal antibodies. |
| Formulation | 20% (v/v) Ethanol |
| Preservative | No Preservative |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C. Do not freeze. |
Background: Protein A
Additional Protein A Products
Product Documents for Protein A Native Protein
Certificate of Analysis
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Product Specific Notices for Protein A Native Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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Protocols
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FAQs for Protein A Native Protein
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Q: During my master thesis, I am working on a large scale ribosome affinity purification in Trypanosoma brucei. Unfortunately, we have some problems with the present antibody (IgG against protein A, extracted from chicken). Therefore, we are searching a better and more efficient alternative. Ideally it is extracted from guinea pig, rat, mouse or rabbit. Searching suitable antibodies, I found you have some in your range of products. Since we need to work large scale and will need a bigger amount of antibodies the next months, we would like to get the possibility to test the new antibodies first. Therefore I would like to ask for an aliquot for testing. You would really help me finding an good alternative!
A: We unfortunately cannot offer testing samples of our antibodies. I am sorry for any inconvenience. We do have two antibodies to Protein A that meet your host requirements. We fully guarantee our antibodies for all listed species and applications. For any applications and/or species not listed, we offer our Innovators Reward Program. In exchange for a review of your experiment using our product in an untested application or species, we will issue you a credit for the purchase price of the antibody.