Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19882

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Protein O-Fucosyltransferase 1/POFUT1. The exact sequence is proprietary.

Localization

Endoplasmic reticulum

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP2-19882]

Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP2-19882]

Western Blot: POFUT1 Antibody [NBP2-19882] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP2-19882]

Immunocytochemistry/ Immunofluorescence: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP2-19882]

Immunocytochemistry/Immunofluorescence: POFUT1 Antibody [NBP2-19882] - Immunofluorescence analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.
Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP2-19882]

Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP2-19882]

Western Blot: POFUT1 Antibody [NBP2-19882] - Sample (15 ug of whole cell lysate) A: HUVEC 12% SDS PAGE gel, diluted at 1:5000.
Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP2-19882]

Western Blot: Protein O-Fucosyltransferase 1/POFUT1 Antibody [NBP2-19882]

Western Blot: POFUT1 Antibody [NBP2-19882] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.

Applications for Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Protein O-Fucosyltransferase 1/POFUT1

This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Long Name

Protein O‑fucosyltransferase 1/POFUT1

Alternate Names

FucT-1, FUT12, O-Fuc-T, O-FUT, POFUT1, Protein OFucosyltransferase 1

Gene Symbol

POFUT1

UniProt

Additional Protein O-Fucosyltransferase 1/POFUT1 Products

Product Documents for Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Protein O-Fucosyltransferase 1/POFUT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail