Proteome Profiler Human Kidney Biomarker Array Kit
R&D Systems | Catalog # ARY019
Key Product Details
Species
Product Summary for Proteome Profiler Human Kidney Biomarker Array Kit
Kit Summary
To simultaneously detect the relative changes of 38 proteins in a single sample. No specialized equipment is necessary.
- Product Datasheet
- Troubleshooting Guide
- Assays for analytes represented in the Human Kidney Biomarker Array Kit
- Compatible with LI-COR* and chemiluminescence detection
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Urine, cell culture supernatant, and cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any biomarker/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of kidney biomarker bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the Product Datasheet.
Stability and Storage
Store the unopened product at 2° C to 8° C. Do not use past expiration date.
| Simultaneously detect the levels of these kidney related proteins in a single sample. | ||
|---|---|---|
| Adiponectin | Fetuin A | Renin |
| Aminopeptidase N | GRO-alpha/CXCL1 | Resistin |
| Angiotensinogen/Serpin A8 | IL-1ra | SCF |
| Annexin V | IL-6 | Serpin A3 |
| beta2-Microglobulin | IL-10 | TNF-alpha |
| Clusterin | TIM-1/KIM-1/HAVCR | TNF RI |
| CXCL16 | Lipocalin-2/NGAL | Trefoil Factor 3 |
| Cyr61/CCN | MCP-1/CCL2 | Thrombospondin-1 |
| Cystatin C | MMP-9 | TWEAK |
| DPPIV/CD26 | Neprilysin | uPA |
| EGF | PSA/KLK-3 | VCAM-1 |
| EGF R/ErbB1 | RAGE | VEGF-A |
| FABP1/L-FABP | RBP-4 | |
Formulation, Preparation, and Storage
Shipping
Storage
Product Documents for Proteome Profiler Human Kidney Biomarker Array Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Proteome Profiler Human Kidney Biomarker Array Kit
For research use only
Citations for Proteome Profiler Human Kidney Biomarker Array Kit
Powered by Bioz
Customer Reviews for Proteome Profiler Human Kidney Biomarker Array Kit
There are currently no reviews for this product. Be the first to review Proteome Profiler Human Kidney Biomarker Array Kit and earn rewards!
Have you used Proteome Profiler Human Kidney Biomarker Array Kit?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
FAQs for Proteome Profiler Human Kidney Biomarker Array Kit
-
Q: Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
A: The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.