Proteome Profiler Human Kidney Biomarker Array Kit
Proteome Profiler Human Kidney Biomarker Array Kit Summary
To simultaneously detect the relative changes of 38 proteins in a single sample. No specialized equipment is necessary.
- Product Datasheet
- Troubleshooting Guide
- Assays for analytes represented in the Human Kidney Biomarker Array Kit
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Urine, cell culture supernatant, and cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any biomarker/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of kidney biomarker bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the Product Datasheet.
Stability and Storage
Store the unopened product at 2° C to 8° C. Do not use past expiration date.
|Simultaneously detect the levels of these kidney related proteins in a single sample.|
|Annexin V||IL-6||Serpin A3|
|CXCL16||Lipocalin-2/NGAL||Trefoil Factor 3|
Citations for Proteome Profiler Human Kidney Biomarker Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Cell cycle arrest and cell death correlate with the extent of ischemia and reperfusion injury in patients following kidney transplantation - Results of an observational pilot study
Authors: FCF Schmitt, E Salgado, J Friebe, T Schmoch, F Uhle, T Fleming, J Zemva, L Kihm, C Nusshag, C Morath, M Zeier, T Bruckner, A Mehrabi, PP Nawroth, MA Weigand, S Hofer, T Brenner
Transpl. Int., 2018;0(0):. 2018
Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes
Authors: M Mustapic, E Eitan, JK Werner, ST Berkowitz, MP Lazaropoul, J Tran, EJ Goetzl, D Kapogianni
Front Neurosci, 2017;11(0):278. 2017
Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?
The proteinase inhibitors are recommended for cell and tissue lysates only. You do not need them for cell culture supernates.
Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
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