Proteome Profiler Human Kidney Biomarker Array Kit

Contains 4 membranes - each spotted in duplicate with 38 different kidney biomarker antibodies
Catalog # Availability Size / Price Qty
ARY019
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Proteome Profiler Human Kidney Biomarker Array Kit Summary

Kit Summary

To simultaneously detect the relative changes of 38 proteins in a single sample. No specialized equipment is necessary.

 

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Urine, cell culture supernatant, and cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any biomarker/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of kidney biomarker bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the Product Datasheet.

Stability and Storage

Store the unopened product at 2° C to 8° C. Do not use past expiration date.

 

 

Simultaneously detect the levels of these kidney related proteins in a single sample.
Adiponectin Fetuin A Renin
Aminopeptidase N GRO-alpha/CXCL1 Resistin
Angiotensinogen/Serpin A8 IL-1ra SCF
Annexin V IL-6 Serpin A3
beta2-Microglobulin IL-10 TNF-alpha
Clusterin TIM-1/KIM-1/HAVCR TNF RI
CXCL16 Lipocalin-2/NGAL Trefoil Factor 3
Cyr61/CCN MCP-1/CCL2 Thrombospondin-1
Cystatin C MMP-9 TWEAK
DPPIV/CD26 Neprilysin uPA
EGF PSA/KLK-3 VCAM-1
EGF R/ErbB1 RAGE VEGF-A
FABP1/L-FABP RBP-4  

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

Product Datasheets

Reagents Provided

Citations for Proteome Profiler Human Kidney Biomarker Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cell cycle arrest and cell death correlate with the extent of ischemia and reperfusion injury in patients following kidney transplantation - Results of an observational pilot study
    Authors: FCF Schmitt, E Salgado, J Friebe, T Schmoch, F Uhle, T Fleming, J Zemva, L Kihm, C Nusshag, C Morath, M Zeier, T Bruckner, A Mehrabi, PP Nawroth, MA Weigand, S Hofer, T Brenner
    Transpl. Int., 2018;0(0):.  2018
  2. Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes
    Authors: M Mustapic, E Eitan, JK Werner, ST Berkowitz, MP Lazaropoul, J Tran, EJ Goetzl, D Kapogianni
    Front Neurosci, 2017;11(0):278.  2017

FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

View all Proteome Profiler Antibody Array FAQs

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