Proteome Profiler Human Protease Inhibitor Array Kit

Contains 4 membranes - each spotted in duplicate with 32 different protease inhibitor antibodies
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R&D Systems Proteome Profiler Antibody Array Kits
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Citations (1)

Proteome Profiler Human Protease Inhibitor Array Kit Summary

  • The Human Protease Inhibitor Array Kit is a rapid, sensitive, and economical tool to simultaneously detect protease inhibitor differences between samples.
  • The relative expression of 32 human protease inhibitors can be determined without performing numerous immunoprecipitations or Western blots.
  • Each capture and detection antibody was carefully selected using commonly used sample types.
Kit Summary

To simultaneously detect the relative changes of 32 protease inhibiting analytes in a single sample. No specialized equipment is necessary.


General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell and tissue lysates, serum, plasma, urine, saliva, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.


Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8°.


Simultaneously detect the levels of these cell stress-related proteins in a single sample.
APP Lipocalin-1 Serpin F1/PEDF
Cystatin A Lipocalin-2/NGAL Testican 1/SPOCK1
Cystatin B RECK Testican 2/SPOCK2
Cystatin C Serpin A5 TFPI
Cystatin E/M Serpin A8/AGT TFPI-2
EMMPRIN Serpin A9/Centerin TIMP-1
Fetuin B Serpin A12 TIMP-2
HAI-1 Serpin B5/Maspin TIMP-3
HAI-2 Serpin B6 TIMP-4
HE4/WFDC2 Serpin B8 Trappin-2/Elafin
Latexin Serpin E1/PAI-1  


Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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Citation for Proteome Profiler Human Protease Inhibitor Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Decidual macrophage derived MMP3 contributes to extracellular matrix breakdown in spiral artery remodeling in early human pregnancy
    Authors: Y Pan, L Yang, D Chen, H Hou, M Zhang, M Chen, F Ning, Q Lu, M Zhao, L Li, GE Lash
    Journal of reproductive immunology, 2022-02-11;150(0):103494.  2022-02-11


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