Proteome Profiler Human Protease Inhibitor Array Kit
Proteome Profiler Human Protease Inhibitor Array Kit Summary
- The Human Protease Inhibitor Array Kit is a rapid, sensitive, and economical tool to simultaneously detect protease inhibitor differences between samples.
- The relative expression of 32 human protease inhibitors can be determined without performing numerous immunoprecipitations or Western blots.
- Each capture and detection antibody was carefully selected using commonly used sample types.
To simultaneously detect the relative changes of 32 protease inhibiting analytes in a single sample. No specialized equipment is necessary.
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell and tissue lysates, serum, plasma, urine, saliva, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8°.
|Simultaneously detect the levels of these cell stress-related proteins in a single sample.|
|Cystatin A||Lipocalin-2/NGAL||Testican 1/SPOCK1|
|Cystatin B||RECK||Testican 2/SPOCK2|
|Cystatin C||Serpin A5||TFPI|
|Cystatin E/M||Serpin A8/AGT||TFPI-2|
|Fetuin B||Serpin A12||TIMP-2|
Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?
The proteinase inhibitors are recommended for cell and tissue lysates only. You do not need them for cell culture supernates.
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