Recombinant Human PSMB10/MECL1 His Protein

Novus Biologicals | Catalog # NBP1-72454

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all PSMB10/MECL1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 40-273 of Human PSMB10/MECL1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTTIAGLVFQ DGVILGADTR ATNDSVVADK SCEKIHFIAP KIYCCGAGVA ADAEMTTRMV ASKMELHALS TGREPRVATV TRILRQTLFR YQGHVGASLI VGGVDLTGPQ LYGVHPHGSY SRLPFTALGS GQDAALAVLE DRFQPNMTLE AAQGLLVEAV TAGILGDLGS GGNVDACVIT KTGAKLLRTL SSPTEPVKRS GRYHFVPGTT AVLTQTVKPL TLELVEETVQ AMEVE

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

26.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PSMB10/MECL1 His Protein

SDS-PAGE: Recombinant Human PSMB10/MECL1 His Protein [NBP1-72454]

SDS-PAGE: Recombinant Human PSMB10/MECL1 His Protein [NBP1-72454]

SDS-Page: Recombinant Human PSMB10/MECL1 His Protein [NBP1-72454] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBP1-72454
Formulation 20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 0.1 M NaCl
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMB10/MECL1

PSMB10 is a member of the proteasome B-type family, also known as the T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Expression of the PSMB10 gene is induced by gamma interferon, and PSMB10 replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. Recombinant human PSMB10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 10

Alternate Names

LMP10, MECl-1, MECL1, Proteasome subunit beta-2i

Gene Symbol

PSMB10

Additional PSMB10/MECL1 Products

Product Documents for Recombinant Human PSMB10/MECL1 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human PSMB10/MECL1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
T Cell Antigen Recognition

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