PSMD12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-29923

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PSMD12 Primary Antibodies »

Product Specifications

Immunogen

Between 406 and 456

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSMD12 Antibody - BSA Free

PSMD12 Antibody

Immunoprecipitation:PSMD12 AntibodyNBP3-29923] -

Immunoprecipitation:PSMD12 AntibodyNBP3-29923] -Detection of human PSMD12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSMD12 antibody NBP3-29923 used for IP at 6 µg/mg lysate. PSMD12 was less efficiently immunoprecipitated by rabbit anti-PSMD12., which recognizes an upstream epitope. For blotting immunoprecipitated PSMD12, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PSMD12 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PSMD12

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. [provided by RefSeq]

Alternate Names

26S proteasome non-ATPase regulatory subunit 12, 26S proteasome regulatory subunit p55, 26S proteasome regulatory subunit RPN5, MGC75406, p55, proteasome (prosome, macropain) 26S subunit, non-ATPase, 12, Rpn5

Gene Symbol

PSMD12

Additional PSMD12 Products

Product Documents for PSMD12 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PSMD12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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