PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73708

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E7

Format

Azide and BSA Free
View all PTK7/CCK4 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PTK7(NP_690619) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free

Western Blot: PTK7/CCK4 Antibody (OTI2E7)Azide and BSA Free [NBP2-73708]

Western Blot: PTK7/CCK4 Antibody (OTI2E7)Azide and BSA Free [NBP2-73708]

Western Blot: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCK4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCK4.
Immunocytochemistry/ Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708]

Immunocytochemistry/ Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708]

Immunocytochemistry/Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CCK4.
Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708]

Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708]

Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CCK4 antibody, and then analyzed by flow cytometry.
Western Blot: PTK7/CCK4 Antibody (OTI2E7)Azide and BSA Free [NBP2-73708]

Western Blot: PTK7/CCK4 Antibody (OTI2E7)Azide and BSA Free [NBP2-73708]

Western Blot: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CCK4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Applications for PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:200-500

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTK7/CCK4

Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene.

Long Name

Protein Tyrosine Kinase 7

Alternate Names

CCK4

Gene Symbol

PTK7

Additional PTK7/CCK4 Products

Product Documents for PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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