Pyruvate Kinase Antibody (OTI1H4)

Novus Biologicals | Catalog # NBP3-44636

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1H4
View all Pyruvate Kinase Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PKLR (NP_000289) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Pyruvate Kinase Antibody (OTI1H4)

Pyruvate Kinase Antibody (OTI1H4) Flow Cytometry;Pyruvate Kinase Antibody (OTI1H4)[NBP3-44636]-

Flow Cytometry;Pyruvate Kinase Antibody (OTI1H4)[NBP3-44636]-

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PKLR antibody, and then analyzed by flow cytometry.
Pyruvate Kinase Antibody (OTI1H4) Immunocytochemistry/ Immunofluorescence;Pyruvate Kinase Antibody (OTI1H4)[NBP3-44636]-

Immunocytochemistry/ Immunofluorescence;Pyruvate Kinase Antibody (OTI1H4)[NBP3-44636]-

Anti-PKLR mouse monoclonal antibody immunofluoreaining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR.
Pyruvate Kinase Antibody (OTI1H4) Western Blot;Pyruvate Kinase Antibody (OTI1H4)[NBP3-44636]-

Western Blot;Pyruvate Kinase Antibody (OTI1H4)[NBP3-44636]-

Western blot analysis of extracts (30ug per lane) from 2 different cell lines lysates by using anti-PKLR antibody.
Pyruvate Kinase Antibody (OTI1H4) Western Blot;Pyruvate Kinase Antibody (OTI1H4)[NBP3-44636]-

Western Blot;Pyruvate Kinase Antibody (OTI1H4)[NBP3-44636]-

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human PKLR plasmid (, hPKLR), mouse PKLR plasmid (MR215886, mPKLR) using anti-PKLR antibody (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody.
Pyruvate Kinase Antibody (OTI1H4) Flow Cytometry;Pyruvate Kinase Antibody (OTI1H4)[NBP3-44636]-

Flow Cytometry;Pyruvate Kinase Antibody (OTI1H4)[NBP3-44636]-

Flow cytometric Analysis of Jurkat cells, using anti-PKLR antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Applications for Pyruvate Kinase Antibody (OTI1H4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pyruvate Kinase

Alternate Names

PKM, PKM2

Gene Symbol

Pyruvate Kinase

Additional Pyruvate Kinase Products

Product Documents for Pyruvate Kinase Antibody (OTI1H4)

Certificate of Analysis

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Product Specific Notices for Pyruvate Kinase Antibody (OTI1H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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