Q: We purchased this protein from you guys and I need its sequence.

A: NBC1-18504: full length protein, aa 1-215.

Recombinant Human Rab5a Protein

Novus Biologicals | Catalog # NBC1-18504

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Applications

SDS-PAGE

View all Rab5a Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-215 of Human Rab5a

Source: E.coli

Amino Acid Sequence: MASRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY DITNEESFAR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS YADDNSLLFM ETSAKTSMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD LTEPTQPTRN QCCSN

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Rab5a Protein

SDS-PAGE: Recombinant Human Rab5a Protein [NBC1-18504]

SDS-Page: Recombinant Human Rab5a Protein [NBC1-18504] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation, and Storage

NBC1-18504

Formulation	20mM MES buffer (pH6.0), 10% glycerol, 0.1mM PMSF
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab5a

Rab5a is a member of RAS oncogene family. The small GTPase, Rab5a is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex. Recombinant human Rab5a was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

RAB5A, member RAS oncogene family, RAB5RAS-associated protein RAB5A, ras-related protein Rab-5A

Gene Symbol

RAB5A

Additional Rab5a Products

Product Documents for Recombinant Human Rab5a Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Rab5a Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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