Rad9 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-193

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rad9 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein of full length human Rad9 protein. [UniProt# Q99638]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad9 Antibody - BSA Free

Western Blot: Rad9 Antibody [NB100-193]

Western Blot: Rad9 Antibody [NB100-193]

Western Blot: Rad9 Antibody [NB100-193] - Analysis of Rad9 on HeLa whole cell extracts using NB100-193.

Applications for Rad9 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:100
Application Notes
This Rad9 antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. By Western blot a band is seen at approximately 42 kDa, however multiple bands may be seen between ~40-60 kDa, depending on phosphorylation of the protein.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad9

Rad9 (cell cycle checkpoint control protein RAD9A or hRAD9) is an evolutionarily conserved protein which serves multiple functions for preserving genomic integrity, and besides its role as intra-S/G2 checkpoint, it also implicates in homologous recombination repair, base excision repair and mismatch repair pathways. PRKCD mediated Rad9 phosphorylation is essential for the assembly of 9-1-1 cell-cycle checkpoint response complex which is recruited to DNA lesion upon damage by RFC (RAD17-replication factor C) clamp loader complex. Thereafter, Rad9 acts as sliding clamp platform on DNA for multiple proteins involved in LP-BER (long-patch base excision repair). 9-1-1 complex stimulates DNA polymerase beta activity/stability, and is necessary for the recruitment of RHNO1 to sites of DNA-DSBs that occurs during S phase of cell cycle. Moreover, Rad9 exerts 3'->5' double stranded DNA exonuclease activity, and it transactivate p53 target genes such as p21 and has been shown to induce apoptosis through its interaction with Bcl-2/Bcl-xL.

Alternate Names

cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, EC 3.1.11.2, hRAD9, RAD9, RAD9 (S. pombe) homolog, RAD9 homolog A (S. pombe)

Gene Symbol

RAD9A

Additional Rad9 Products

Product Documents for Rad9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Rad9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rad9 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Rad9 Antibody - BSA Free

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  • Q: Is there any possibility that Novus would make a ChIP grade antibody? Can you tell me how should I test it, just cross-link and precipitated, then check if there are some DNAs that I am interested?

    A: This antibody has not been tested in ChIP. If you are interested in testing the antibody we have an Innovators Reward Program where we reward our customers for analyzing our antibodies in species and applications that are previously untested.

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