Raji Whole Cell Lysate

Human

SDS-PAGE, Western Blot

Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents. Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Raji whole cell extract lysate

Cell

Blood Lymph

Whole

1X Laemmli Sample Buffer [62.5mM Tris HCl, 25% Glycerol, 2% SDS, 0.01% bromophenol blue, pH 6.8]

2.5 mg/ml

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Store at -80C. Avoid freeze-thaw cycles.