Ran Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31776

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Xenopus

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Drosophila (95%), Rat (100%), Rhesus Macaque (100%), Sheep (100%), Zebrafish (97%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ran Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ran. The exact sequence is proprietary.

Reactivity Notes

Frog (100%).

Localization

Nucleus, Cytoplasm, Melanosome |

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ran Antibody - BSA Free

Western Blot: Ran Antibody [NBP1-31776]

Western Blot: Ran Antibody [NBP1-31776]

Western Blot: Ran Antibody [NBP1-31776] - Mouse tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAN antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Ran Antibody [NBP1-31776]

Immunocytochemistry/ Immunofluorescence: Ran Antibody [NBP1-31776]

Immunocytochemistry/Immunofluorescence: Ran Antibody [NBP1-31776] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: Ran Antibody [NBP1-31776]

Western Blot: Ran Antibody [NBP1-31776]

Western Blot: Ran Antibody [NBP1-31776] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE; antibody diluted at 1:1000.

Applications for Ran Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-31776 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ran

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Alternate Names

Androgen receptor-associated protein 24, ARA24Gsp1, GTPase Ran, GTP-binding nuclear protein Ran, guanosine triphosphatase Ran, member RAS oncogene family, OK/SW-cl.81, RAN, member RAS oncogene family, RanGTPase, Ras-like protein TC4, Ras-related nuclear protein, TC4

Gene Symbol

RAN

Additional Ran Products

Product Documents for Ran Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ran Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for Ran Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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  • Ran Antibody
    Name: Ye Zhang
    Application: Western Blot
    Sample Tested: Human melanoma cells, Sample Amount: 25ug
    Species: Human
    Verified Customer | Posted 04/13/2017
    Ran Antibody - BSA Free NBP1-31776

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Protocols

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FAQs

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