Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RanBP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 150 and the C-terminus (residue 201) of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RanBP1 Antibody - BSA Free

Western Blot: RanBP1 Antibody [NB100-79815]

Western Blot: RanBP1 Antibody [NB100-79815]

Western Blot: RanBP1 Antibody [NB100-79815] - Detection of human RanBP1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RanBP1 (NB100-79815). Detection: DAB
Immunoprecipitation: RanBP1 Antibody [NB100-79815]

Immunoprecipitation: RanBP1 Antibody [NB100-79815]

Immunoprecipitation: RanBP1 Antibody [NB100-79815] - Detection of human RanBP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RanBP1 antibody NB100-79815 (lot 2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RanBP1 was also immunoprecipitated by the previous lot (lot 1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A and B)

Applications for RanBP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RanBP1

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Alternate Names

HTF9A, MGC88701, RAN binding protein 1, Ran-binding protein 1, RanBP1, ran-specific GTPase-activating protein

Entrez Gene IDs

5902 (Human)

Gene Symbol

RANBP1

UniProt

NP_002873.1 (Human)

Additional RanBP1 Products

Product Documents for RanBP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RanBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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