RAP1GAP Antibody (8V9I6)

Name: RAP1GAP Antibody (8V9I6)
Brand: Novus Biologicals
SKU: NBP3-16234
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16234

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8V9I6 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rap1GAP (P47736). MIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFPLILLPQFGGYWIEGTNHEITSIPETEPLQSPTTKVKLECNPTARIYRKHFLGKEHF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAP1GAP Antibody (8V9I6)

Western Blot: RAP1GAP Antibody (8V9I6) [NBP3-16234]

Western Blot: RAP1GAP Antibody (8V9I6) [NBP3-16234] - Western blot analysis of extracts of various cell lines, using RAP1GAP Rabbit mAb (NBP3-16234) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunocytochemistry/ Immunofluorescence: RAP1GAP Antibody (8V9I6) [NBP3-16234]

Immunocytochemistry/Immunofluorescence: RAP1GAP Antibody (8V9I6) [NBP3-16234] - Immunofluorescence analysis of NIH-3T3 cells using RAP1GAP Rabbit mAb (NBP3-16234) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for RAP1GAP Antibody (8V9I6)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAP1GAP

Rap1GAP, an 85/95kDa protein, is a member of a family of GAP's specific for Rap1 (1). Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain (2-4). Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis (5).

Alternate Names

GTPase activating protein 1, RAP1 GTPase activating protein

Gene Symbol

RAP1GAP

Additional RAP1GAP Products

Product Documents for RAP1GAP Antibody (8V9I6)

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Certificate of Analysis

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Product Specific Notices for RAP1GAP Antibody (8V9I6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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