RAPGEF3 Antibody (5A7K7)

Name: RAPGEF3 Antibody (5A7K7)
Brand: Novus Biologicals
SKU: NBP3-16343
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16343

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5A7K7 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 824-923 of human RAPGEF3 (O95398). FIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAPGEF3 Antibody (5A7K7)

Western Blot: RAPGEF3 Antibody (5A7K7) [NBP3-16343]

Western Blot: RAPGEF3 Antibody (5A7K7) [NBP3-16343] - Western blot analysis of extracts of various cell lines, using RAPGEF3 Rabbit mAb (NBP3-16343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry-Paraffin: RAPGEF3 Antibody (5A7K7) [NBP3-16343]

Immunohistochemistry-Paraffin: RAPGEF3 Antibody (5A7K7) [NBP3-16343] - Immunohistochemistry of paraffin-embedded human placenta using RAPGEF3 Rabbit mAb (NBP3-16343) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for RAPGEF3 Antibody (5A7K7)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAPGEF3

RAPGEF3, also known as Rap guanine nucleotide exchange factor 3, is a 104 kDa 923 amino acid protein with two shorter isoforms produced by alternative splicing. The gene is necessary for rearranging actin at cell to cell junctions. RAPGEF3 is currently being studied for its involvement with leukemia, melanoma, pancreatitis, thyroiditis, carcinoma, prostatitis and neuronitis. RAPGEF3 is linked to the chemotaxis, cell adhesion, insulin secretion, and immune system pathways where it interacts with GNAI2, GNAI1, PDE3B, RAP1A and RAP1B.

Long Name

Rap guanine nucleotide exchange factor 3

Alternate Names

cAMP-GEFI, CGEF1, EPAC, EPAC 1, EPAC1

Gene Symbol

RAPGEF3

Additional RAPGEF3 Products

Product Documents for RAPGEF3 Antibody (5A7K7)

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Certificate of Analysis

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Product Specific Notices for RAPGEF3 Antibody (5A7K7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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