RASEF Antibody (S03-1G6)
Novus Biologicals | Catalog # NBP3-19968
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S03-1G6 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide of human RASEF (Uniprot # Q8IZ41)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RASEF Antibody (S03-1G6)
Western Blot: RASEF Antibody (S03-1G6) [NBP3-19968]
Western Blot: RASEF Antibody (S03-1G6) [NBP3-19968] - Western blot detection of RASEF in Jurkat cell lysates using NBP3-19968 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 83kDa.Immunocytochemistry/Immunofluorescence: RASEF Antibody (S03-1G6) [NBP3-19968] -
Immunocytochemistry/Immunofluorescence: RASEF Antibody (S03-1G6) [NBP3-19968] - Immunocytochemistry of RASEF (green) in Hela using NBP3-19968 at dilution 1/20, and DAPI (blue)Applications for RASEF Antibody (S03-1G6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1/20
Western Blot
1/1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RASEF
Alternate Names
FLJ31614, RAB45, RAB45, member RAS oncogene family, RAS and EF hand domain containing, RAS and EF-hand domain containing, ras and EF-hand domain-containing protein, Ras-related protein Rab-45
Gene Symbol
RASEF
Additional RASEF Products
Product Documents for RASEF Antibody (S03-1G6)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RASEF Antibody (S03-1G6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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