Rat mGluR2/3 Antibody

(1 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Rat ~110 kDa mGluR2 and mGluR3 monomers and ~220 kDa homodimers
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Peptide from the C-terminus region of rat mGluR2 and mGluR3
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of mGluR2/3 by Western Blot Western blot of rat hippocampal lysate showing the specific immunolabeling of the ~110 kDa monomer and the ~220 kDa dimer of mGluR2 and mGluR3.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: mGluR2/3
Rat metabotropic glutamate receptor 2 (mGluR2; also mGlu2) is a 95 kDa, 854 amino acid (aa), 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. Group II receptors include mGluR2 and mGluR3. Both are presynaptic, considered to negatively regulate the release of glutamate, associate with Gi/Go proteins, and inhibit adenylate cyclase, thus decreasing cAMP concentration. The effects of mGluR2 and 3 are not always identical. On microglia, mGluR2 but not mGluR3 stimulation induces TNF-alpha release. mGluR2 is believed to exist as a dimer. Dimerization presumably creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. The cytoplasmic tails of rat mGluR2 and mGluR3 are 64% aa identical; over the last 18 aa, they are 95% aa identical.
  • References:
    1. Rudd, M.T. and J.A. Mccauley (2005) Curr. Top. Med. Chem. 5:869.
    2. Gasparini, F. et al. (2002) Curr. Opin. Pharmacol. 2:43.
    3. Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
    4. Shen, K-Z. and S.W. Johnson (2003) J. Physiol. 533:489.
    5. Taylor, D.L. et al. (2005) J. Neurosci. 25:2952.
  • Long Name:
    Metabotropic Glutamate Receptor 2 and 3
  • Alternate Names:
    GLUR2; glutamate metabotropic receptor 2; glutamate receptor, metabotropic 2; GPRC1Bmetabotropic glutamate receptor 2; mGlu2; mGluR2; mGluR2/3; MGLUR2glutamate receptor homolog
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. mGluR5 in the nucleus accumbens is critical for promoting resilience to chronic stress.
    Authors: Shin S, Kwon O, Kang J, Kwon S, Oh S, Choi J, Kim C, Kim D
    Nat Neurosci, 2015;18(7):1017-24.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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