Rat TCR  gamma /δ APC-conjugated Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat TCR gamma /δ in flow cytometry.
  • Source
    Monoclonal Mouse IgG1 Clone # V65
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    CD3+ TCR alpha / beta - rat T cell hybridoma line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TCR  gamma /δ in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Anti-Rat CD4 Fluorescein‑conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat TCR  gamma /δ APC‑conjugated Monoclonal Antibody (Catalog # FAB6089A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TCR gamma/delta
TCR gamma /δ is associated with CD3 and expressed on a subset of T cells in the thymus, spleen, lymph node, and intestinal epithelium.
  • References:
    1. Kuhnlein, P. et al. (1994) J. Immunol. 153:979.
  • Entrez Gene IDs:
    915 (Human); 12500 (Mouse); 25710 (Rat)
  • Alternate Names:
    CD3d; delta polypeptide; T cell receptor gamma locus; T3D; T-cell antigen receptor, gamma polypeptide; T-cell rearranging gene, gamma; T-cell receptor, gamma cluster; TCR gamma/delta; TCRG; TiT3; TRG
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 8  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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