RBM3 Antibody (JE63-84)

Immunohistochemistry: RBM3 Antibody (JE63-84) [NBP3-32899]

Immunohistochemistry: RBM3 Antibody (JE63-84) [NBP3-32899] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-RBM3 antibody (NBP3-32899) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32899) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: RBM3 Antibody (JE63-84) [NBP3-32899]

Immunocytochemistry/ Immunofluorescence: RBM3 Antibody (JE63-84) [NBP3-32899] - Immunocytochemistry analysis of Hela cells labeling RBM3 with Rabbit anti-RBM3 antibody (NBP3-32899) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RBM3 antibody (NBP3-32899) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta III tubulin (M0805-8, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: RBM3 Antibody (JE63-84) [NBP3-32899]

Flow Cytometry: RBM3 Antibody (JE63-84) [NBP3-32899] - Flow cytometric analysis of THP-1 cells labeling RBM3.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32899, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).