Recombinant Amphibian TGF-beta 5 Protein

Carrier Free

Catalog # Availability Size / Price Qty
245-B5-002/CF
245-B5-010/CF

With Carrier

Catalog # Availability Size / Price Qty
245-B5-002
245-B5-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (1)
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Recombinant Amphibian TGF-beta 5 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.01-0.03 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived amphibian TGF-beta 5 protein
Gly271-Ser382 & Gln274-Ser382
Accession #
N-terminal Sequence
Analysis

Gly271 & Gln274

Predicted Molecular Mass
12.6 kDa

Product Datasheets

245-B5 (with carrier)

245-B5/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

245-B5

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution

Reconstituted at 20 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

245-B5/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution

Reconstitute at 20 μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 5

TGF-beta 5 is a member of the TGF-beta family of growth factors. These proteins are stable, multifunctional factors with a wide variety of effects on the growth and differentiation of virtually all cell types. These actions on growth or differentiation may be stimulatory or inhibitory, depending on the cell type, growth conditions, state of differentiation and on the presence of other growth factors. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. The in vivo role of TGF-beta 5 is also not known, but the presence of this factor in Xenopus embryos, tadpole cells, and adult tissues suggests a role in the mediation of differentiation and development. Similar roles have been suggested for other members of the TGF-beta family. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

Long Name
Transforming Growth Factor beta 5
Entrez Gene IDs
397778 (Amphibian)
Alternate Names
TGFbeta 5; TGF-beta 5

Citation for Recombinant Amphibian TGF-beta 5 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein.
    Authors: Mogi K, Goto M, Ohno E, Azumi Y, Takeuchi S, Toyoizumi R
    Int. J. Dev. Biol., 2003;47(1):15-29.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In Vivo

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