Recombinant Amphibian TGF-beta 5 Protein

R&D Systems | Catalog # 245-B5

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Amphibian TGF-beta 5 Protein (245-B5)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

P16176

Applications

Bioactivity
View all TGF-beta 5 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived amphibian TGF-beta 5 protein
Gly271-Ser382 & Gln274-Ser382

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly271 & Gln274

Predicted Molecular Mass

12.6 kDa

Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389.
The ED50 for this effect is 0.01-0.03 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 245-B5
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution

Reconstituted at 20 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 245-B5/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution

Reconstitute at 20 μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta 5

TGF-beta 5 is a member of the TGF-beta family of growth factors. These proteins are stable, multifunctional factors with a wide variety of effects on the growth and differentiation of virtually all cell types. These actions on growth or differentiation may be stimulatory or inhibitory, depending on the cell type, growth conditions, state of differentiation and on the presence of other growth factors. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. The in vivo role of TGF-beta 5 is also not known, but the presence of this factor in Xenopus embryos, tadpole cells, and adult tissues suggests a role in the mediation of differentiation and development. Similar roles have been suggested for other members of the TGF-beta family. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

Long Name

Transforming Growth Factor beta 5

Alternate Names

TGFbeta 5

Entrez Gene IDs

397778 (Amphibian)

Gene Symbol

TGFB1.L

UniProt

P16176

Additional TGF-beta 5 Products

Product Documents for Recombinant Amphibian TGF-beta 5 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 245-B5 (with carrier)
Download SDS 245-B5/CF (carrier free)

Product Specific Notices for Recombinant Amphibian TGF-beta 5 Protein

Triton is a registered trademark of Union Carbide Corp.

For research use only

Citations for Recombinant Amphibian TGF-beta 5 Protein

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