Recombinant Chicken Caronte Fc Chimera Protein, CF

R&D Systems | Catalog # 1051-CA

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Key Product Details

  • R&D Systems NS0-derived Recombinant Chicken Caronte Fc Chimera Protein (1051-CA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_990154

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity, Bioactivity
View all Caronte Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived chicken Caronte protein
Chicken Caronte
(Asp16 - Asn272) and
(Glu18 - Asn272)
Accession # NP_990154		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp16 & Glu18

Predicted Molecular Mass

56 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to cause heart reversals when injected into the right lateral plate of a population of Xenopus neurulae embryos.
80 ng per embryo induces 25-50% heart reversals.

Measured by its binding ability in a functional ELISA.
Immobilized rchCaronte/Fc Chimera at 5 µg/mL (100 µL/well) can bind hBMP-4 with a linear range of 0.8-100 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 1051-CA in the following applications:

Formulation, Preparation, and Storage

1051-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caronte

Chicken Caronte, the ortholog of mammalian Cerberus, is a member of the Cerberus/DAN family of secreted glycoproteins. There are at least 5 Dan Domain (DAND) family members including DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), Cerberus (DAND4), and Coco (DAND5). Caronte is expressed in the left paraxial mesoderm and is the molecule responsible for transducing left-sided positional information from the node to the periphery of the lateral plate mesoderm (LPM) during chicken embryonic development. Caronte functions as a BMP antagonist, inducing Nodal and downstream genes in the LPM by interfering with the repressive activity of bilaterally-produced BMPs. In addition, antagonism of BMPs by Caronte induces the expression of the Lefty gene in the midline. Caronte binds both BMP-4 and BMP-7 in immunoprecipitation experiments. Recombinant chicken Caronte preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA and induce heart reversals in Xenopus embryos.

References

  1. Pearce, J. et al. (1999) Dev. Biol. 209:98.
  2. Yokouchi, Y. et al. (1999) Cell. 98:573.
  3. King, T. and N. Brown (1999) Nature 401:222.
  4. Ahu, L. et al. (1999) Curr. Biol. 9:931.
  5. Rodriguez-Esteban, C. et al. (1999) Nature 401:243.

Alternate Names

Chicken Caronte

Entrez Gene IDs

395623 (Chicken)

Gene Symbol

CER1

UniProt

NP_990154

Additional Caronte Products

Product Documents for Recombinant Chicken Caronte Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Chicken Caronte Fc Chimera Protein, CF

For research use only

Related Research Areas

BMP Family

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    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 02/18/2021
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