Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF

Catalog # Availability Size / Price Qty
9246-CD-100
Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF Bioactivity
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Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Fc gamma  RIIB/CD32b is immobilized on a His Tag Antibody coated plate, it binds Biotinylated Human IgG. The concentration of Biotinylated Human IgG that produces 50% of the optimal binding response is approximately 0.3-1.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey Fc gamma RIIB/CD32b protein
Ala46-Pro217, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala46
Predicted Molecular Mass
20 kDa
SDS-PAGE
21-35 kDa, reducing conditions

Product Datasheets

9246-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Cynomolgus Monkey Fc gamma RIIB Protein, CF Bioactivity View Larger

When Recombinant Cynomolgus Monkey Fc gamma RIIB/CD32b(Catalog # 9246-CD) is immobilized on a His Tag Antibody coated plate,Biotinylated Human IgG binds with an ED50 of
0.3-1.5 μg/mL.

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Background: Fc gamma RIIB/CD32b

Receptors for the Fc region of IgG (Fc gamma  Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI/CD64, Fc gamma  RII/CD32, and Fc gamma  RIII/CD16. CD32 proteins are encoded by three genes in human (Fc gamma RII A, B, and C), two in cynomolgus (Fc gamma RII A and B), and one in mouse (Fc gamma RIIB). Fc gamma RII A and C contain activating cytoplasmic ITAM sequences, while Fc gamma RIIB contains an inhibitory cytoplasmic ITIM sequence. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1). The approximately 40 kDa mature cynomolgus Fc gamma RIIB consists of a 175 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 51 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RIIB shares 90%, 64%, and 61% aa sequence identity with human, mouse, and rat Fc gamma RIIB, respectively. It binds to both cynomolgus and human IgG subclasses 1-4 (2). Fc gamma RIIB is expressed on B cells, monocytes, dendritic cells, neutrophils, mast cells, and basophils (1). Ligation of Fc gamma RIIB triggers signaling that suppresses B cell expansion, plasma cell differentiation, production of autoimmune rheumatoid factors, and down-regulation of TLR4 with reduced LPS responsiveness (3-6).

References
  1. Nimmerjahn, F. and J.V. Ravetch (2008) Nat. Rev. Immunol. 8:34.
  2. Warncke, M. et al. (2012) J. Immunol. 188:4405.
  3. Takai, T. et al. (1996) Nature 379:346.
  4. Karnell, J.L. et al. (2014) J. Immunol. 192:1480.
  5. Moll, T. et al. (2004) J. Immunol. 173:4724.
  6. Zhang, Y. et al. (2009) J. Immunol. 182:554.
Long Name
Fc gamma Receptor II
Entrez Gene IDs
2213 (Human); 14130 (Mouse); 289211 (Rat); 102139530 (Cynomolgus Monkey)
Alternate Names
CD32 antigen; CD32b; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIB; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; FCGR2B; FcgRIIB; FCRIIB; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b

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