Recombinant Cynomolgus Monkey FCAR/CD89 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human IgA is immobilized at 5 μg/mL, 100 μL/well, the concentration of Recombinant Cynomolgus Monkey FCAR/CD89 that produces 50% of the optimal binding response is 0.5-2.5 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Gln22-Asn227, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No reults obtained. Gln 22 inferred from enzymatic pyroglutamate treatment revealing Glu23
  • Predicted Molecular Mass
    24 kDa
  • SDS-PAGE
    33-70 kDa, reducing conditions
9516-FA
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Human IgA iscoated at 5 µg/mL (100 μL/well), Recombinant Cynomolgus MonkeyFCAR/CD89 (Catalog # 9516-FA) binds with an ED50 of 0.5-2.5 μg/mL.
Background: FCAR/CD89
FCAR, also called Fc alpha RI or CD89, is a variably glycosylated 50-100 kDa myeloid-specific type I transmembrane (TM) Fc receptor for IgA and is a member of the multi-chain immune recognition receptor (MIRR) family (1-3). Cynomolgus FCAR is predicted to contain a 21 amino acid (aa) signal sequence and 206 aa extracellular (ECD), 19 aa TM and 41 aa cytoplasmic domains (4). The Arg230 within the TM domain of human FCAR supports interaction with the ITAM-containing signaling subunit, FcR gamma, which contains a TM Asp (5-7). Two ECD C2-type Ig-like domains (EC1 and 2) are oriented at right angles (8). Up to two molecules of FCAR can bind one molecule of serum IgA via EC1 (8). Many human FCAR splice variants have been reported, but only two have been identified as proteins (9, 10). The a.2 form, which lacks 22 aa just prior to the TM domain, is exclusively expressed in alveolar macrophages. The a.3 form lacks EC2. FCAR binds monomeric, polymeric and secretory IgA, but does not mediate the barrier function of secretory IgA in mucosal epithelium (1-3). Shedding and circulation of polymeric IgA/FCAR immune complexes has been reported (11). Circulating neutrophils, eosinophils, and monocytes express FCAR (12). Tissue expression of FCAR is mainly from neutrophils; FCAR is down-regulated as monocytes differentiate to tissue macrophages (12). On neutrophils, a significant amount of FCAR lacks FcR gamma, but can still be endocytosed to early endosomes and recycled to the cell surface (5). Binding of serum IgA to FCAR is transient and anti-inflammatory, inhibiting IgG or IgE-induced degranulation (6). Sustained aggregation of FCAR results in inflammatory responses (6). FcR gamma signaling is required for these and for transport to late endosomes (5-7). Within ECD, Cynomolgus FCAR shows 83% aa identity with human FCAR. No ortholog occurs in mouse. FCAR structure resembles the KIR/ILT/LIR/MIR family more than other IgA receptors, including pIgR, Fc alpha /μR, asialoglycoprotein receptor (ASGR1) and transferrin receptor (TfR) (1-3).
  • References:
    1. Wines, B. D. and P. M. Hogarth (2006) Tissue Antigens 68:103.
    2. Otten, M. A. and M. van Egmon (2004) Immunol. Lett. 92:23.
    3. Montiero, R. C. and J. G. J. van de Winkel (2003) Annu. Rev. Immunol. 21:177.
    4. Maliszewski, C. R. et al. (1990) J. Exp. Med. 172:1665.
    5. Launay, P. et al. (1999) J. Biol. Chem. 274:7216.
    6. Pasquier, B. et al. (2005) Immunity 22:31.
    7. Shen, L. et al. (2001) Blood 97:205.
    8. Herr, Y. et al. (2003) Nature 423:614.
    9. Patry, C. et al. (1996) J. Immunol. 156:4442.
    10. Togo, S. et al. (2003) FEBS Lett. 535:205.
    11. van der Boog, P. J. M. et al. (2002) J. Immunol. 168:1252.
    12. Hamre, R. et al. (2003) Scand. J. Immunol. 57:506.
  • Long Name:
    IgA Fc Receptor
  • Entrez Gene IDs:
    2204 (Human); 365183 (Rat); 102145896 (Cynomolgus Monkey)
  • Alternate Names:
    CD89 antigen; CD89; CD89IgA Fc receptor; Fc alpha receptor; Fc fragment of IgA, receptor for; FCAR; immunoglobulin alpha Fc receptor
Related Research Areas
Primary Antibodies
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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His Tag Alexa Fluor® 594-conjugated Antibody

ICFlow IC0501T
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His Tag Alexa Fluor® 405-conjugated Antibody

ICFlow IC050V
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His Tag Alexa Fluor® 647-conjugated Antibody

ICFlow IC050R
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC0501N
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His Tag Alexa Fluor® 405-conjugated Antibody

ICFlow IC0501V
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His Tag Alexa Fluor® 750-conjugated Antibody

ICFlow IC0501S
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His Tag Alexa Fluor® 750-conjugated Antibody

ICFlow IC050S
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His Tag Alexa Fluor® 350-conjugated Antibody

ICFlow IC0501U
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His Tag Alexa Fluor® 594-conjugated Antibody

ICFlow IC050T
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His Tag Alexa Fluor® 647-conjugated Antibody

ICFlow IC0501R
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC0501G
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His Tag Alexa Fluor® 350-conjugated Antibody

ICFlow IC050U
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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