Recombinant Cynomolgus Monkey IgG1 Fc Protein, CF

R&D Systems | Catalog # 9315-HG

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Cynomolgus Monkey IgG1 Fc Protein (9315-HG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

AAF14057

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey IgG1 protein
Pro110-Lys333

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro110, and Ala-Leu-Ala-Pro110

Predicted Molecular Mass

25 kDa

SDS-PAGE

30-40 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey IgG1 Fc is immobilized at 0.15 µg/mL (100 µL/well), the concentration of Recombinant Human Fc gamma  RI/CD64 (Catalog # 1257-FC) that produces 50% of the optimal binding response is 0.2-1.2 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey IgG1 Fc Protein, CF

Recombinant Cynomolgus Monkey IgG1 Fc Protein Bioactivity

Recombinant Cynomolgus Monkey IgG1 Fc Protein Bioactivity

When Recombinant Cynomolgus Monkey IgG1 Fc (Catalog # 9315-HG) is coated at 0.15 µg/mL, 100 µL/well, Recombinant Human Fc gamma RI/CD64 (Catalog # 1257-FC) binds with an ED50 of 0.2-1.2 ng/mL.

Formulation, Preparation, and Storage

9315-HG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG1

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in human adult blood. It is composed of two heavy chains and two light chains. The variability of the IgG pool is generated by somatic recombination in B cells that have been exposed to foreign material. IgA, IgG, IgM, IgD, and IgD classes of human immunoglobulin (as well as the isotypes IgG1, IgG2a, IgG2b, and IgG3) are generated by class switch recombination within the heavy chain locus (1). Recombination within the light chain locus selects either kappa or lambda chains. Antibodies are secreted by plasma cells of the B lymphocyte lineage and constitute the humoral component of adaptive immunity (2, 3). Their variable regions bind to foreign antigens, while their Fc regions are recognized by Fc receptors expressed on macrophages, monocytes, neutrophils, NK cells, and CD8+ cytotoxic T cells (4). Phagocyte uptake of antibody-antigen complexes and antibody-opsonized pathogens is critical for clearance of the foreign material and immune homeostasis.

References

  1. Stavnezer, J. et al. (2008) Annu. Rev. Immunol. 26:261.
  2. Nutt, S.L. et al. (2015) Nat. Rev. Immunol. 15:160.
  3. Swanson, C.L. et al. (2013) Immunol. Res. 55:277.
  4. Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

UniProt

AAF14057

Additional IgG1 Products

Product Documents for Recombinant Cynomolgus Monkey IgG1 Fc Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus Monkey IgG1 Fc Protein, CF

For research use only

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