Recombinant Cynomolgus Monkey IL-27 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10307-IL-025
Recombinant Cynomolgus Monkey IL-27 His-tag Protein, CF Data
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Recombinant Cynomolgus Monkey IL-27 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED50 for this effect is 0.75-6.0 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IL-27 protein
Cynomolgus Monkey EBI-3
(Arg36-Lys244)
Accession # XP_005587614.1
GSGSSRGGSGSGGSGGGGSKL

Cynomolgus Monkey IL-27a

*Unique sequence

6-His tag
N-terminusC-terminus

* Unique Sequence: This gene was isolated from a cynomolgus monkey cDNA library. The closest match to the obtained sequence is a genomic prediction for rhesus monkey, Accession XP_028697194.1 (Phe42-Pro254, A170T).
N-terminal Sequence
Analysis
Arg36
Predicted Molecular Mass
50 kDa
SDS-PAGE
54-65 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10307-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50for this effect is 0.75-6.0 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey IL-27 His-tag (Catalog # 10307-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-65 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-27

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 50 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, a 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The Cynomolgous EBI3 gene encodes a 244 amino acid (aa) precursor that contains a 36 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Cynomolgous monkey EBI3 is 94% aa identical to Human EBI3. The Cynomolgous monkey p28 gene encodes a 254 aa precursor that contains a 41 aa signal sequence and 214 aa mature regions. The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Cynomolgous monkey p28 is 92% aa identical to human p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (7). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130 (6, 8, 9). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory cytokine, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 7). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (10).

References
  1. Boulay, J-L. et al. (2003) Immunity 19:159.
  2. Trinchieri, G. et al. (2003) Immunity 19:641.
  3. Murakami, M. et al. (2004) Growth Factors 22:75.
  4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715.
  5. Devergne, O. et al. (1996) J. Virology 70:1143.
  6. Pflanz, S. et al. (2002) Immunity 16:779.
  7. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  8. Pflanz, S. et al. (2004) J Immunol 172:2225.
  9. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  10. Holscher, C. (2004) Med. Microbiol. Immunol. 193:1.
Long Name
Interleukin 27
Entrez Gene IDs
246778 (Human); 246779 (Mouse); 102121242 (Cynomolgus Monkey)
Alternate Names
IL-27 p28 subunit; IL-27 subunit alpha; IL27; IL-27; IL27A; IL-27-A; IL27-A; IL27p28; IL30; interleukin 27; interleukin 30; interleukin-27 subunit alpha; MGC71873; p28IL-27A

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