Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein, CF

R&D Systems | Catalog # 10530-L6

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Key Product Details

  • R&D Systems CHO-derived Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein (10530-L6)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_001270930.1

Applications

Bioactivity
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Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Ly6E protein
Cynomolgus Monkey Ly6E
(Leu21-Ser101)
Accession # NP_001270930.1		 3x GSL  Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu21

Predicted Molecular Mass

35 kDa

SDS-PAGE

38-50 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect 1-10 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein Bioactivity

Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein Bioactivity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.
Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein (Catalog # 10530-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-50 kDa and 76-100 kDa, respectively.

Formulation, Preparation, and Storage

10530-L6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ly6E

Lymphocyte antigen 6E (Ly6E), also known as Stem cell antigen 2 (SCA-2) or Thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 protein superrfamily (1, 2). There are at least twenty different human Ly6 proteins that have been identified, and they are either secreted or GPI-anchor membrane-bound proteins (3). Cynomolgus Ly6E is synthesized as a precursor molecule that includes a signal peptide, a Ly6E chain, and a propeptide. Within the mature, main chain region, cynomolgus Ly6E shares 94% amino acid sequence identity with human Ly6E. Ly6E is involved in cell signalling transduction, cell adhesion, immune regulation and drug resistance, and is over-expressed in human malignancies, including head and neck squamous cell carcinomas and lung, and oesophageal cancers (4-7). Moreover, Ly6E over-expresses in gastric cancer cells, and is important for cell survival, proliferation and migration, and could be a novel oncogenic protein for efficient diagnosis and treatment of gastric cancer (2). LY6E has recently been shown to promote viral entry of some flaviviruses, including West Nile virus, Dengue virus, and Zika virus (8). Our in-house data indicate that Ly6E inhibits the human T cell activation, including IL-2, IFN-g secretions.

References

  1. Gumley, T.P. et al. (1995) Immunol. Cell Biol. 73:277.
  2. Lv, Y. et al. (2018) Cell. Physiol. Biochem. 45:1219.
  3. Kong, H.K. and J.H. Park. (2012) BMB Rep. 45:595.
  4. Luo, L. et al. (2016) Oncotarget 7:11165.
  5. Yeom, C.J. et al. (2016) Oncotarget 7:65837.
  6. AlHossiny, M. et al. (2016) Cancer Res. 76:3376.
  7. Asundi, J. et al. (2015) Clin Cancer Res. 21:3252.
  8. Mar, K.B. et al. (2018) Nat Commun. 9:3603.

Long Name

Lymphocyte Antigen 6 Family Member E

Alternate Names

Ly-6E, RIGE, SCA2, TSA1

Entrez Gene IDs

4061 (Human); 17069 (Mouse); 362934 (Rat); 101866361 (Cynomolgus Monkey)

Gene Symbol

LY6E

UniProt

NP_001270930.1

Additional Ly6E Products

Product Documents for Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein, CF

For research use only

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