Recombinant Cynomolgus Monkey Tie-2 His-tag Protein, CF Summary
Ala23-Lys745, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 1 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Cynomolgus Monkey Tie-2 His-tag (Catalog # 10341-T2) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 (Catalog # 623-AN) binds with an ED50of 1.5-12 ng/mL.
2 μg/lane of Recombinant Cynomolgus Monkey Tie-2 His-tag (Catalog # 10341-T2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 96-107 kDa.
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region (1). These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis (2).
Cynomolgus Monkey Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with a 22 residue putative signal peptide, a 723 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects (3).
- Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
- Takakura, N. et al. (1998) Immunity 9:677.
- Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
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