Recombinant Human Angiopoietin-2 Protein

Formulations:
Catalog # Availability Size / Price Qty
623-AN-025
Recombinant Human Angiopoietin-2 Protein Binding Activity
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Product Details
Citations (38)
FAQs
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Recombinant Human Angiopoietin-2 Protein Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to activate Tie-2 in C6 rat glial cells transfected with human Tie-2. 0.2 μg/mL of Recombinant Human Angiopoietin‑2 significantly induces phosphorylation of human Tie-2. Measured by its binding ability in a functional ELISA. When Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin‑2 (Catalog # 623-AN) binds with an ED50 of 0.5-4 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Angiopoietin-2 protein
Tyr19-Phe496, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Tyr19
Structure / Form
Oligomer
Predicted Molecular Mass
56 kDa
SDS-PAGE
66 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

623-AN

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, CHAPS and PEG with BSA as a carrier protein and Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

623-AN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, CHAPS and PEG. *1 MG pack size is supplied as a 0.2 µm filtered solution in MOPS, NaCl, CHAPS and PEG.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *1 MG pack size is shipped with dry ice or equivalent. Upon receipt, store it immediately at -70 °C.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

Data Image

Binding Activity View Larger

When Recombinant Human Tie-2 Fc Chimera (Catalog # 313-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 (Catalog # 623-AN) binds with an ED50of 0.5-4 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-2

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation (1, 2). Mature Ang-2 is 478 amino acids (aa) in length. It contains one coiled‑coil domain (aa 166-248) that mediates multimerization, and a C-terminal fibrinogen-like domain (aa 275-495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang-2 is 65-66 kDa in size. Under non‑reducing conditions, both natural and recombinant Ang-2 form 140 kDa dimers, 200 kDa trimers, and 250-300 kDa tetramers and pentamers (3-6). Alternate splicing generates a short isoform that lacks 52 amino acids (aa) preceding the coiled‑coil domain (4). Mature human Ang-2 shares 86% aa sequence identity with mouse and rat Ang-2. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus (3). It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies (1, 3, 7). Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2 (2). While Ang-1 is a potent Tie‑2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang-2 becomes as a Tie-2 agonist (3, 8‑11). The short isoform appears to block the binding of either Ang-1 or full-length Ang-2 to Tie-2 (4). Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression (12, 13). Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells (8, 13, 14). Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development (7, 15). It mediates the up‑regulation of ICAM‑1 and VCAM‑1 on EC, which facilitates the adhesion of leukocytes during inflammation (16). Ang-2 is up‑regulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue (17-20). Its direct interaction with Integrins promotes tumor cell invasion (21, 22). Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells (20).

References
  1. Augustin, H.G. et al. (2009) Nat. Rev. Mol. Cell Biol. 10:165.
  2. Murdoch, C. et al. (2007) J. Immunol. 178:7405.
  3. Maisonpierre, P.C. et al. (1997) Science 27:55.
  4. Kim, I. et al. (2000) J. Biol. Chem. 275:18550.
  5. Procopio, W.N. et al. (1999) J. Biol. Chem. 274:30196.
  6. Kim, K-T. et al. (2005) J. Biol. Chem. 280:20126.
  7. Gale, N.W. et al. (2002) Dev. Cell 3:411.
  8. Yuan, H.T. et al. (2009) Mol. Cell. Biol. 29:2011.
  9. Falcon, B.L. et al. (2009) Am. J. Pathol. 175:2159.
  10. Kim, H-Z. et al. (2009) Biochim. Biophys. Acta 1793:772.
  11. Kim, I. et al. (2001) Cardiovasc. Res. 49:872.
  12. Lobov, I.B. et al. (2002) Proc. Natl. Acad. Sci. 99:11205.
  13. Cao, Y. et al. (2007) Cancer Res. 67:3835.
  14. Nasarre, P. et al. (2009) Cancer Res. 69:1324.
  15. Dellinger, M. et al. (2008) Dev. Biol. 319:309.
  16. Fiedler, U. et al. (2006) Nat. Med. 12:235.
  17. Koga, K. et al. (2001) Cancer Res. 61:6248.
  18. Etoh, T. et al. (2001) Cancer Res. 61:2145.
  19. Tressel, S.L. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1989.
  20. Liu, X.S. et al. (2009) J. Biol. Chem. 284:22680.
  21. Hu, B. et al. (2006) Cancer Res. 66:775.
  22. Imanishi, Y. et al. (2007) Cancer Res. 67:4254.
Entrez Gene IDs
285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine)
Alternate Names
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand

Citations for Recombinant Human Angiopoietin-2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Rapid Development of Glaucoma Via ITV Nonselective ANGPT 1/2 Antibody: A Potential Role for ANGPT/TIE2 Signaling in Primate Aqueous Humor Outflow
    Authors: EA Thackaberr, Y Zhou, CL Zuch de Za, G Fuh, CV Lee, S Sanowar, JB Ridgway, AM Kusi, C Farman, H Booler, D Sheinson, CA Rasmussen, PE Miller, E Wakshull, M Yan, V Bantseev
    Invest. Ophthalmol. Vis. Sci., 2019;60(13):4097-4108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration
    Authors: J Kim, JR Park, J Choi, I Park, Y Hwang, H Bae, Y Kim, W Choi, JM Yang, S Han, TY Chung, P Kim, Y Kubota, HG Augustin, WY Oh, GY Koh
    Sci Adv, 2019;5(2):eaau6732.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. IL-8 associates with a pro-angiogenic and mesenchymal subtype in glioblastoma
    Authors: S Conroy, FAE Kruyt, M Wagemakers, KPL Bhat, WFA den Dunnen
    Oncotarget, 2018;9(21):15721-15731.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Novel inhibitors of the cellular RAS components, Poricoic acids, target Smad3 phosphorylation and Wnt/β-catenin pathway against renal fibrosis
    Authors: M Wang, DQ Chen, L Chen, G Cao, H Zhao, D Liu, ND Vaziri, Y Guo, YY Zhao
    Br. J. Pharmacol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Angiopoietin-2 promotes extracellular matrix degradation in human degenerative nucleus pulposus cells
    Authors: K Wang, L Kang, W Liu, Y Song, X Wu, Y Zhang, W Hua, K Zhao, S Li, J Tu, R Luo, C Yang
    Int. J. Mol. Med., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina
    Authors: H Elamaa, M Kihlström, E Kapiainen, M Kaakinen, I Miinalaine, S Ragauskas, M Cerrada-Gi, S Mering, M Nätynki, L Eklund
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA
  7. Angiopoietin-2 promotes osteogenic differentiation of thoracic ligamentum flavum cells via modulating the Notch signaling pathway
    Authors: X Yang, Z Chen, X Meng, C Sun, M Li, L Shu, D Fan, T Fan, AY Huang, C Zhang
    PLoS ONE, 2018;13(12):e0209300.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP
    Authors: T Souma, BR Thomson, S Heinen, IA Carota, S Yamaguchi, T Onay, P Liu, AK Ghosh, C Li, V Eremina, YK Hong, AN Economides, D Vestweber, KG Peters, J Jin, SE Quaggin
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Endothelial Tie1-mediated angiogenesis and vascular abnormalization promote tumor progression and metastasis
    Authors: S La Porta, L Roth, M Singhal, C Mogler, C Spegg, B Schieb, X Qu, RH Adams, HS Baldwin, S Savant, HG Augustin
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Angiopoietins bind thrombomodulin and inhibit its function as a thrombin cofactor
    Authors: C Daly, X Qian, C Castanaro, E Pasnikowsk, X Jiang, BR Thomson, SE Quaggin, N Papadopoul, Y Wei, JS Rudge, G Thurston, GD Yancopoulo, S Davis
    Sci Rep, 2018;8(1):505.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities
    Authors: SS Lavoie, E Dumas, B Vulesevic, PE Neagoe, M White, MG Sirois
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration
    Authors: K Wang, W Liu, Y Song, X Wu, Y Zhang, S Li, Y Gao, J Tu, Y Liu, C Yang
    Lab. Invest., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Inhibition of VEGF and Angiopoietin-2 to Reduce Brain Metastases of Breast Cancer Burden
    Authors: KA Bohn, CE Adkins, MI Nounou, PR Lockman
    Front Pharmacol, 2017;8(0):193.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling
    Authors: N Morooka, S Futaki, R Sato-Nishi, M Nishino, Y Totani, C Shimono, I Nakano, H Nakajima, N Mochizuki, K Sekiguchi
    Circ. Res, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Tie1 controls angiopoietin function in vascular remodeling and inflammation
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells
    Authors: Wilson Chan
    PLoS ONE, 2016;11(6):e0156994.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. New Insights into the Pro-Inflammatory Activities of Ang1 on Neutrophils: Induction of MIP-1? Synthesis and Release
    PLoS ONE, 2016;11(9):e0163140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Association of Angiopoietin-2 and Ki-67 Expression with Vascular Density and Sunitinib Response in Metastatic Renal Cell Carcinoma
    Authors: J Rautiola, A Lampinen, T Mirtti, A Ristimäki, H Joensuu, P Bono, P Saharinen
    PLoS ONE, 2016;11(4):e0153745.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  19. Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival
    Authors: J Kloepper, L Riedemann, Z Amoozgar, G Seano, K Susek, V Yu, N Dalvie, RL Amelung, M Datta, JW Song, V Askoxylaki, JW Taylor, C Lu-Emerson, A Batista, ND Kirkpatric, K Jung, M Snuderl, A Muzikansky, KG Stubenrauc, O Krieter, H Wakimoto, L Xu, LL Munn
    Proc Natl Acad Sci USA, 2016;113(16):4476-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1
    Sci Adv, 2016;2(4):e1501290.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Insertion of scFv into the hinge domain of full-length IgG1 monoclonal antibody results in tetravalent bispecific molecule with robust properties
    Authors: Binyam Bezabeh
    MAbs, 2016;0(0):0.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  22. Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
    Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
    Nat Commun, 2015;6(0):5962.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironment.
    Authors: Brunckhorst M, Xu Y, Lu R, Yu Q
    Am J Pathol, 2014;184(8):2285-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils.
    Authors: Haddad L, Sirois M
    PLoS ONE, 2014;9(2):e88980.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.
    Authors: Keats E, Dominguez J, Grant M, Khan Z
    Stem Cells, 2014;32(6):1649-60.
    Species: Human
    Sample Types: Protein
    Applications: Standard
  26. Directed evolution of an angiopoietin-2 ligand trap by somatic hypermutation and cell surface display.
    Authors: Brindle, Nicholas, Sale, Julian E, Arakawa, Hiroshi, Buerstedde, Jean-Mar, Nuamchit, Teonchit, Sharma, Shikha, Steele, Kathryn
    J Biol Chem, 2013;288(46):33205-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Structural basis for angiopoietin-1-mediated signaling initiation.
    Authors: Yu, Xuehong, Seegar, Tom C M, Dalton, Annamari, Tzvetkova-Robev, Dorothea, Goldgur, Yehuda, Rajashankar, Kanagala, Nikolov, Dimitar, Barton, William
    Proc Natl Acad Sci U S A, 2013;110(18):7205-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2010;84(3):239-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis.
    Authors: Brunckhorst MK, Wang H, Lu R, Yu Q
    Cancer Res., 2010;70(18):7283-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Sensitive and multiplexed detection of proteomic antigens via quantum dot aggregation.
    Authors: Soman C, Giorgio T,
    Nanomedicine, 2009;5(4):402-9.
    Species: N/A
    Sample Types: N/A
    Applications: Quantum Dot
  31. Quantum dot self-assembly for protein detection with sub-picomolar sensitivity.
    Authors: Soman CP, Giorgio TD,
    Langmuir, 2008;24(8):4399-404.
    Species: N/A
    Sample Types: N/A
    Applications: Quantum Dot
  32. Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts.
    Authors: Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K
    Nat. Cell Biol., 2008;10(5):527-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells.
    Authors: Vart RJ, Nikitenko LL, Lagos D, Trotter MW, Cannon M, Bourboulia D, Gratrix F, Takeuchi Y, Boshoff C
    Cancer Res., 2007;67(9):4042-51.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Control)
  34. Obligatory participation of macrophages in an angiopoietin 2-mediated cell death switch.
    Authors: Rao S, Lobov IB, Vallance JE, Tsujikawa K, Shiojima I, Akunuru S, Walsh K, Benjamin LE, Lang RA
    Development, 2007;134(24):4449-58.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  35. Intraovarian actions of anti-angiogenic agents disrupt periovulatory events during the menstrual cycle in monkeys.
    Authors: Xu F, Hazzard TM, Evans A, Charnock-Jones S, Smith S, Stouffer RL
    Contraception, 2005;71(4):239-48.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: In Vivo
    Applications: In Vivo
  36. Expansion of human SCID-repopulating cells under hypoxic conditions.
    Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
    J. Clin. Invest., 2003;112(1):126-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.
    Authors: Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger M, Wu D, Min W
    Nat Med, 0;22(9):1033-1042.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Angiopoietin-2 as a Biomarker and Target for Immune Checkpoint Therapy.
    Authors: Wu X, Giobbie-Hurder A, Liao X, Connelly C, Connolly E, Li J, Manos M, Lawrence D, McDermott D, Severgnini M, Zhou J, Gjini E, Lako A, Lipschitz M, Pak C, Abdelrahman S, Rodig S, Hodi F
    Cancer Immunol Res, 0;5(1):17-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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