Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF

Fc Chimera
Catalog # Availability Size / Price Qty
10287-N1-100
Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF Binding Activity
2 Images
Product Details
FAQs
Reviews

Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 His-tag (Catalog # 3064-N3) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera (Catalog # 10287-N1) binds with an ED50 of 2.5-20 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived Nectin-1 protein
Cynomolgus Monkey/Rhesus Macaque Nectin-1
(Gln31-Gly349)
Accession # XP_005579992.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln31 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
62 kDa
SDS-PAGE
87-101 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10287-N1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity View Larger

When Recombinant Human Nectin-3 (Catalog # 3064-N3) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera (Catalog # 10287-N1) binds with an ED50of 2.5-20 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 87-100 kDa and 170-200 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Nectin-1

Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means "to connect", indicating the role of nectins in Ca2+-independent cell-cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). Based on the similarity with human Nectin-1, the 519 amino acid (aa) cynomolgus Nectin-1 may contain a 30 aa signal sequence, a 328 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 140 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding, and two C2-type (3,4). The ECD of cynomolgus Nectin-1 shares 98% and 100% aa identity with human and rhesus monkey Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4 - 7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8 - 11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14). In colorectal cancer, Nectin-1 expression was found to be associated with a high risk for early disease recurrence (15).

References
  1. Lopez, M. et al. (1995) Gene 155:261.
  2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
  3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
  4. Lopez, M. et al. (2001) J. Virol. 75:5684.
  5. Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.
  6. Linehan, M. M. et al. (2004) J. Virol. 78:2530.
  7. Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.
  8. Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.
  9. Togashi, H. et al. (2006) J. Cell Biol. 174:141.
  10. Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.
  11. Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.
  12. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
  13. Suzuki, K. et al. (2000) Nat. Genet. 25:427.
  14. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  15. Tampakis, A. et al. (2019) Oncology. 96:318.
Long Name
Poliovirus Receptor Related 1
Entrez Gene IDs
5818 (Human); 58235 (Mouse); 102144697 (Cynomolgus Monkey)
Alternate Names
CD111 antigen; CD111; CLPED1ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgRPVRR; HVEC; HVECpoliovirus receptor-like 1; MGC142031; Nectin1; Nectin-1; OFC7nectin 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; PVRR1nectin-1; SK-12

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF and earn rewards!

Have you used Recombinant Cynomolgus/Rhesus Macaque Nectin-1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review