Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF

Catalog # Availability Size / Price Qty
6415-GH-020
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Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to hydrolyze 4-methylumbelliferyl-alpha -D-galactopyranoside. The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
Source
E. coli-derived e. coli alpha-Galactosidase/a-Gal protein
Val2-Val708, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Analysis
Met  
Predicted Molecular Mass
82 kDa
SDS-PAGE
70-75 kDa, reducing conditions

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Background: alpha-Galactosidase/a-Gal

alpha -Galactoside is prevalent in animals and plants. The alpha -Galactosidase ( alpha -Gal) from Escherichia coli is a useful tool for the removal of alpha 1,3-linked and alpha 1,6‑linked galactosides from the non-reducing terminus of complex carbohydrates and glycoproteins (1). alpha -Gal efficiently hydrolyzes raffinose, an alpha -D-galactosylsucrose, to D‑galactose and sucrose. The enzyme can also hydrolyze other alpha -Galactosides such as melibiose, stachyose, verbascose, and galactinol. The enzyme does not cleave beta -linked galactose, such as lactose.

References
  1. Schmid, K. and Schmitt, R. (1976) Eur. J. Biochem. 67:95.
  2. Spangenberg, P. et al. (2000) Carbohydr. Res. 329:65.
Entrez Gene IDs
5589615 (E. coli)
Alternate Names
alphaGalactosidase; alpha-Galactosidase; rafA

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