Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF
R&D Systems | Catalog # 6415-GH
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Key Product Details
- R&D Systems E. coli-derived Recombinant E. coli alpha-Galactosidase/a-Gal Protein (6415-GH)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived e. coli alpha-Galactosidase/a-Gal protein
Val2-Val708, with an N-terminal Met and 6-His tag
Val2-Val708, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
82 kDa
SDS-PAGE
70-75 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze 4-methylumbelliferyl-alpha -D-galactopyranoside.
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation, and Storage
6415-GH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: alpha-Galactosidase A/GLA
References
- Schmid, K. and Schmitt, R. (1976) Eur. J. Biochem. 67:95.
- Spangenberg, P. et al. (2000) Carbohydr. Res. 329:65.
Alternate Names
Agalsidase alpha, GALA, Melibiase
Entrez Gene IDs
2717 (Human)
Gene Symbol
GLA
UniProt
Additional alpha-Galactosidase A/GLA Products
Product Documents for Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF (6415-GH):
Materials
- Assay Buffer: 100 mM MES, pH 6.5
- Recombinant E. coli alpha ‑Galactosidase/ alpha ‑Gal (rE. coli alpha -Gal) (Catalog # 6415-GH)
- Substrate: 4-Methylumbelliferyl-alpha -D-galactopyranoside (Sigma, Catalog # M7633), 6.7 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rE. coli alpha -Gal to 0.5 ng/μL in Assay Buffer.
- Dilute Substrate to 800 μM in Assay Buffer.
- Load into a plate 50 μL of 0.5 ng/μL rE. coli alpha -Gal, and start the reaction by adding 50 μL of 800 μM Substrate. For Substrate Blanks, load 50 μL of Assay Buffer and 50 μL of 800 μM Substrate.
- Read plate at excitation and emission wavelengths of 365 nm and 445 nm, respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 4-Methylumbelliferone (4-MU) (Sigma, Catalog # M1381).
- rE. coli alpha -Gal: 0.025 μg
- Substrate: 400 μM
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