As a heat shock protein and a serine protease, HTRA (Protease Do or the DegP protease) is indispensable for bacterial survival at temperatures above 42 °C (1). It is a periplasmic protein with a signal peptide of 26 amino acid residues. The mature protein contains two 90 amino acid PDZ domains, which start at residue 280. The serine protease activity is inhibited by diisopropyl fluorophosphate or dichloroisocoumarin. Four human proteins have been identified that are homologous to E. coli HTRA and believed to play a role in arthritis, cell growth, unfolded stress response, apoptosis, aging, and tumor progression (2).
Recombinant E. coli HTRA/Protease Do Protein, CF
R&D Systems | Catalog # 1670-SE
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Key Product Details
- R&D Systems E. coli-derived Recombinant E. coli HTRA/Protease Do Protein (1670-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived e. coli HTRA/Protease Do protein
Ala27-Gln474, with a C-terminal 10 His tag and ERSIGS sequence
Ala27-Gln474, with a C-terminal 10 His tag and ERSIGS sequence
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala27
Predicted Molecular Mass
49 kDa
SDS-PAGE
50 kDa, 41 kDa and 27 kDa, reducing conditions
Activity
Measured by its ability to cleave beta -casein.
Recombinant E. coli HTRA/Protease Do will cleave >95% cleavage of beta -casein, as measured under the described conditions.
Recombinant E. coli HTRA/Protease Do will cleave >95% cleavage of beta -casein, as measured under the described conditions.
Formulation, Preparation, and Storage
1670-SE
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: HTRA/Protease Do
References
- Skorko-Glonek, J. et al. (1995) J. Biol. Chem. 270:11140.
- Clausen, T. et al. (2002) Mol. Cell 10:443.
Long Name
High Temperature Requirement
Alternate Names
DegP, Protease Do
Entrez Gene IDs
962111 (E. coli)
Gene Symbol
THRB
UniProt
Additional HTRA/Protease Do Products
Product Documents for Recombinant E. coli HTRA/Protease Do Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant E. coli HTRA/Protease Do Protein, CF
For research use only
Related Research Areas
Citations for Recombinant E. coli HTRA/Protease Do Protein, CF
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Protocols
View specific protocols for Recombinant E. coli HTRA/Protease Do Protein, CF (1670-SE):
Materials
- Assay Buffer: 50 mM Tris, pH 8.0
- Recombinant E.coli HTRA/Protease Do (reHTRA) (Catalog # 1670-SE)
- beta -Casein (Sigma, Catalog # C-6905), 1.0 mg/mL stock in 25 mM Tris, 0.15 M NaCl, pH 7.5
- SDS-PAGE with silver staining
- Dilute beta -Casein to 0.4 mg/mL in Assay Buffer.
- Dilute reHTRA to 0.04 mg/mL in Assay Buffer.
- Combine 25 μL of beta -Casein and 25 μL of reHTRA in a tube. Prepare a control containing 25 μL of beta -Casein and 25 μL of Assay Buffer.
- Incubate the mixture for 1 hour at 45 °C.
- Stop the reaction by adding SDS-PAGE sample buffer.
- Analyze the cleavage by SDS-PAGE using silver staining to detect protein.
- reHTRA: 0.02 mg/mL
- beta -Casein: 0.2 mg/mL
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