Recombinant Feline IL-15 Protein

Carrier Free

Catalog # Availability Size / Price Qty
7805-FL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
7805-FL-010
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Feline IL-15 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.2-1.2 ng/mL.
Source
E. coli-derived feline IL-15 protein
Asn49-Ser162
Accession #
N-terminal Sequence
Analysis
Asn49
Structure / Form
Monomer
Predicted Molecular Mass
13 kDa
SDS-PAGE
10 kDa, reducing conditions

Product Datasheets

7805-FL (with carrier)

7805-FL/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7805-FL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7805-FL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1). Mature feline IL-15 shares 80%, 83%, 68%, and 68% amino acid sequence identity with canine, human, mouse, and rat IL-15, respectively (2). IL-15 binds with high affinity to IL-15 R alpha (3). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (4). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (5). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (6). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (5). In human and mouse, soluble IL-15-binding forms of IL‑15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (7‑9). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (3, 8). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (10). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells (11‑15). An important component of these functions is the ability of IL-15 to induce dendritic cell differentiation and inflammatory activation (10, 13). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells (16). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (17).

References
  1. De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  2. Dean, G.A. et al. (2005) Cytokine 29:77.
  3. Giri, J.G. et al. (1995) EMBO J. 14:3654.
  4. Giri, J. et al. (1994) EMBO J. 13:2822.
  5. Dubois, S. et al. (2002) Immunity 17:537.
  6. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
  7. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  8. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  9. Bergamaschi, C. et al. (2012) Blood 120:e1.
  10. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  11. Mortier, E. et al. (2008) J. Exp. Med. 205:1213.
  12. Gordy, L.E. et al. (2011) J. Immunol. 187:6335.
  13. Harris, K.M. (2011) J. Leukoc. Biol. 90:727.
  14. Xia, J. et al. (2010) Clin. Immunol. 134:130.
  15. Schluns, K.S. et al. (2002) J. Immunol. 168:4827.
  16. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529.
  17. Barra, N.G. et al. (2010) Obesity 18:1601.
Long Name
Interleukin 15
Entrez Gene IDs
3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names
IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

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