Recombinant Human Activin AB Protein

R&D Systems | Catalog # 1066-AB

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Activin AB Protein (1066-AB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_002183 ( beta A) & NP_002184 ( beta B)

Structure / Form

Disulfide-linked heterodimer

Applications

Bioactivity
View all Activin AB Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Activin AB protein
Human Activin beta A
(Gly311 - Ser426)
Accession # NP_002183.1
Human Activin beta B
(Gly293 - Ala407)
Accession # NP_002184.2
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly311( beta A subunit) & Gly293 ( beta B subunit)

Predicted Molecular Mass

12.8 kDa ( beta A subunit) and 13 kDa ( beta B subunit)

SDS-PAGE

14 kDa, reducing conditions

Activity

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340.
The ED50 for this effect is 0.2‑1.2 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 1066-AB in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1066-AB
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1066-AB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution Reconstitute at 100 µg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Activin AB

Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Activin proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and roles in reproductive physiology. Activins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned. The nomenclature reflects the subunit composition of the proteins: Activin A ( beta A - beta A), Activin B ( beta B - beta B), and Activin AB ( beta A - beta B). Activin A, Activin B, and Activin AB are present in gonadal tissues and are biologically active proteins. However, little is known about the contribution of the other beta subunits to Activin formation and function since knock-outs of beta C and beta E in mice do not exhibit a phenotype.

At the amino acid sequence level, the mature human beta A subunit is 100% identical to mouse beta A, while the mature human and mouse beta B subunits share 98% identity. The mature beta A and beta B subunits share less than 80% amino acid identity. Mice with targeted mutations of beta A, beta B, or both genes do not show mesodermal defects, indicating Activin is not involved in mesoderm formation in mammals as it is in Xenopus. Also, the double homozygous mutants have the whole spectrum of defects associated with either mutation alone, suggesting that Activin beta A and beta B do not compensate for one another, nor do they have overlapping functions. Similar to other TGF-beta family members, Activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type II. Activin binds directly to ACT RII, the complex then associates with ACT RI and initiates signaling through the SMADs.

References

  1. Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
  2. Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.
  3. Chang, H. et al. (2001) Mol. Cell. Endocrinology 180:39.

UniProt

NP_002183 ( beta A) & NP_002184 ( beta B)

Additional Activin AB Products

Product Documents for Recombinant Human Activin AB Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 1066-AB (with carrier)
Download SDS 1066-AB/CF (carrier free)

Product Specific Notices for Recombinant Human Activin AB Protein

For research use only

Citations for Recombinant Human Activin AB Protein

Customer Reviews for Recombinant Human Activin AB Protein (1)

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    Name: Jeffrey Frost
    Application: SDS-PAGE Control
    Verified Customer | Posted 07/09/2020
    Recombinant Human Activin AB Protein 1066-AB

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