Recombinant Human ADAM15 Protein, CF

Catalog # Availability Size / Price Qty
9234-AD-050
ADAM15 Protein Bioactivity
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Recombinant Human ADAM15 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.5-3 μg/mL
Source
Chinese Hamster Ovary cell line, CHO-derived human ADAM15 protein
Asp207-Ser693 with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Asp207
Predicted Molecular Mass
53 kDa
SDS-PAGE
67-79 kDa, reducing conditions

Product Datasheets

9234-AD

Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity ADAM15 Protein Bioactivity View Larger

Immobilized RecombinantHuman ADAM15 (Catalog # 9234-AD)supports theadhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effectis 0.5-3 μg/mL.

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Background: ADAM15

A disintegrin and metalloproteinase 15 (ADAM15), also known as MDC15 and Metargin, is an approximately 110 kDa transmembrane member of the M12B family of peptidases (1). Human ADAM15 is synthesized with a 189 amino acid (aa) propeptide that contains a cysteine switch motif. The 75 kDa activated form of human ADAM15 (after removal of the propeptide) consists of a 490 aa extracellular domain (ECD) with a peptidase, disintegrin, cysteine-rich, and EGF-like domain followed by a 21 aa transmembrane segment and a 146 aa cytoplasmic domain (2). Within the ECD, human ADAM15 shares 85% aa sequence identity with mouse and rat ADAM15. Alternative splicing generates multiple additional isoforms with various deletions or substitutions in the cytoplasmic domain, deletion in the propeptide and peptidase domains, or truncation at the beginning of the EGF-like domain (3, 4). ADAM15 is widely expressed, including on colonic epithelial cells, smooth muscle cells, vascular endothelial cells, and it is upregulated during chronic inflammation and tumor progression (5-8). It is also expressed on spermatocytes where both propeptide and the protease domain are cleaved during spermatocyte maturation (9, 10). ADAM15 promotes endothelial permeability, T cell adhesion, MMP-9 production and activation, and tumor cell metastasis (6, 8, 11, 12). It binds to Integrin  alpha V  beta 3 (13) and mediates the proteolytic shedding of cell surface
N-Cadherin, E-Cadherin, MICB, CD23/Fc epsilon  RII, and FGF R2 (IIb) (6, 7, 14-16).

References
  1. Edwards, D.R. et al. (2008) Mol. Aspects Med. 29:258.
  2. Kratzschmar, J. et al. (1996) J. Biol. Chem. 271:4593.
  3. Kleino, I. et al. (2009) J. Cell. Biochem. 108:877.
  4. Kleino, I. et al. (2007) BMC Mol. Biol. 8:90.
  5. Mosnier, J.F. et al. (2006) Lab. Invest. 86:1064.
  6. Najy, A.J. et al. (2008) Cancer Res. 68:1092.
  7. Duan, X. et al. (2013) Mol. Med. Rep. 7:991.
  8. Dong, D.D. et al. (2015) Oncol. Rep. 34:2451.
  9. Pasten-Hidalgo, K. et al. (2008) Reproduction 136:41.
  10. Pasten, K. et al. (2014) Reproduction 148:623.
  11. Sun, C. et al. (2010) Cardiovasc. Res. 87:348.
  12. Charrier, L. et al. (2007) J. Biol. Chem. 282:16948.
  13. Zhang, X.P. et al. (1998) J. Biol. Chem. 273:7345.
  14. Najy, A.J. et al. (2008) J. Biol. Chem. 283:18393.
  15. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
  16. Maretzky, T. et al. (2009) Biochem. J. 420:105.
Long Name
A Disintegrin and Metalloprotease-like Domain 15
Entrez Gene IDs
8751 (Human); 11490 (Mouse)
Alternate Names
ADAM 15; ADAM metallopeptidase domain 15; ADAM15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin

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Recombinant Human ADAM15 Protein, CF
By Anonymous on 09/27/2017
Application: Other
Reason for Rating: I got a nice single band on my western bolt.