Recombinant Human ADAM15 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.5-3 μg/mL
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Asp207-Ser693 with a C-terminal 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Asp207
  • Predicted Molecular Mass
    53 kDa
  • SDS-PAGE
    67-79 kDa, reducing conditions
9234-AD
 
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Immobilized RecombinantHuman ADAM15 (Catalog # 9234-AD)supports theadhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effectis 0.5-3 μg/mL.
Background: ADAM15
A disintegrin and metalloproteinase 15 (ADAM15), also known as MDC15 and Metargin, is an approximately 110 kDa transmembrane member of the M12B family of peptidases (1). Human ADAM15 is synthesized with a 189 amino acid (aa) propeptide that contains a cysteine switch motif. The 75 kDa activated form of human ADAM15 (after removal of the propeptide) consists of a 490 aa extracellular domain (ECD) with a peptidase, disintegrin, cysteine-rich, and EGF-like domain followed by a 21 aa transmembrane segment and a 146 aa cytoplasmic domain (2). Within the ECD, human ADAM15 shares 85% aa sequence identity with mouse and rat ADAM15. Alternative splicing generates multiple additional isoforms with various deletions or substitutions in the cytoplasmic domain, deletion in the propeptide and peptidase domains, or truncation at the beginning of the EGF-like domain (3, 4). ADAM15 is widely expressed, including on colonic epithelial cells, smooth muscle cells, vascular endothelial cells, and it is upregulated during chronic inflammation and tumor progression (5-8). It is also expressed on spermatocytes where both propeptide and the protease domain are cleaved during spermatocyte maturation (9, 10). ADAM15 promotes endothelial permeability, T cell adhesion, MMP-9 production and activation, and tumor cell metastasis (6, 8, 11, 12). It binds to Integrin  alpha V  beta 3 (13) and mediates the proteolytic shedding of cell surface
N-Cadherin, E-Cadherin, MICB, CD23/Fc epsilon  RII, and FGF R2 (IIb) (6, 7, 14-16).
  • References:
    1. Edwards, D.R. et al. (2008) Mol. Aspects Med. 29:258.
    2. Kratzschmar, J. et al. (1996) J. Biol. Chem. 271:4593.
    3. Kleino, I. et al. (2009) J. Cell. Biochem. 108:877.
    4. Kleino, I. et al. (2007) BMC Mol. Biol. 8:90.
    5. Mosnier, J.F. et al. (2006) Lab. Invest. 86:1064.
    6. Najy, A.J. et al. (2008) Cancer Res. 68:1092.
    7. Duan, X. et al. (2013) Mol. Med. Rep. 7:991.
    8. Dong, D.D. et al. (2015) Oncol. Rep. 34:2451.
    9. Pasten-Hidalgo, K. et al. (2008) Reproduction 136:41.
    10. Pasten, K. et al. (2014) Reproduction 148:623.
    11. Sun, C. et al. (2010) Cardiovasc. Res. 87:348.
    12. Charrier, L. et al. (2007) J. Biol. Chem. 282:16948.
    13. Zhang, X.P. et al. (1998) J. Biol. Chem. 273:7345.
    14. Najy, A.J. et al. (2008) J. Biol. Chem. 283:18393.
    15. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
    16. Maretzky, T. et al. (2009) Biochem. J. 420:105.
  • Long Name:
    A Disintegrin and Metalloprotease-like Domain 15
  • Entrez Gene IDs:
    8751 (Human); 11490 (Mouse)
  • Alternate Names:
    ADAM 15; ADAM metallopeptidase domain 15; ADAM15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 17  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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