Recombinant Human Afamin His-tag Protein, CF Summary
Leu22-Asn599, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human Afamin (Catalog # 8065-AF) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 71 - 80 kDa and 55 - 70 kDa, respectively.
AFM (Afamin; also known as Alpha -Albumin) is a secreted monomeric glycoprotein of the Alb/Albumin family of molecules. It is expressed by hepatocytes (1), CNS endothelial cells (2) and osteoclasts (3), and circulates in the blood at low μg/mL concentrations. AFM is known to bind and transport vitamin E family molecules, playing an important role for transporting at the blood-brain-barrier (2). It also plays a role in maintaining solubility for transporting Wnt proteins (4, 5). AFM also serves as an osteoclast-derived chemoattractant for preosteoblasts, providing a rational for the observation that bone formation often follows bone resorption (3). Mature human AFM is 578 amino acids (aa) in length (aa 22-599). It contains three consecutive albumin domains (aa 36-206, aa 211-403 and aa 404-599) that contain a characteristic 5 or 6 intrachain disulfide bonds. Full-length human AFM shares 66% aa sequence identity with mouse AFM and 67% aa sequence identity with rat AFM. The importance of Afamin in transport of molecules has led to a suggested diagnostic role in various diseases, including pre-eclampsia (6), ovarian cancer (7), and both gestational and type-2 diabetes (8, 9).
- Liu, H. et al. (2011) DNA Cell Biol. 30:137.
- Kratzer, I. et al. (2009) J Neurochem. 108(3):707.
- Kim, B.J. et al. (2012) Bone. 51:431.
- Naschberger, A. et al. (2017) Structure. 25:1907.
- Mihara, E. et al. (2016) Elife. 5:11621.
- Köninger, A. et al. (2018) Arch Gynecol Obstet. 298(5):1009.
- Aktas B. et al. (2013). Anticancer Res. 33:329.
- Tramontana A. et al. (2018) Clin. Chim. Acta. 476:160.
- Kollerits, B. et al. (2017) Diabetes Care. 40:1386.
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