Recombinant Human Afamin His-tag Protein, CF

Catalog # Availability Size / Price Qty
8065-AF-050
Recombinant Human Afamin His-tag Protein, CF SDS-PAGE
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Recombinant Human Afamin His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) in a functional ELISA. The ED50 for this effect is 0.8-6.4 µg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Afamin protein
Leu22-Asn599, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu22
Predicted Molecular Mass
67 kDa
SDS-PAGE
71-80 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8065-AF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Afamin (Catalog # 8065-AF) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 71 - 80 kDa and 55 - 70 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Afamin

AFM (Afamin; also known as Alpha -Albumin) is a secreted monomeric glycoprotein of the Alb/Albumin family of molecules. It is expressed by hepatocytes (1), CNS endothelial cells (2) and osteoclasts (3), and circulates in the blood at low μg/mL concentrations. AFM is known to bind and transport vitamin E family molecules, playing an important role for transporting at the blood-brain-barrier (2). It also plays a role in maintaining solubility for transporting Wnt proteins (4, 5). AFM also serves as an osteoclast-derived chemoattractant for preosteoblasts, providing a rational for the observation that bone formation often follows bone resorption (3). Mature human AFM is 578 amino acids (aa) in length (aa 22-599). It contains three consecutive albumin domains (aa 36-206, aa 211-403 and aa 404-599) that contain a characteristic 5 or 6 intrachain disulfide bonds. Full-length human AFM shares 66% aa sequence identity with mouse AFM and 67% aa sequence identity with rat AFM. The importance of Afamin in transport of molecules has led to a suggested diagnostic role in various diseases, including pre-eclampsia (6), ovarian cancer (7), and both gestational and type-2 diabetes (8, 9).

References
  1. Liu, H. et al. (2011) DNA Cell Biol. 30:137.
  2. Kratzer, I. et al. (2009) J Neurochem. 108(3):707.
  3. Kim, B.J. et al. (2012) Bone. 51:431.
  4. Naschberger, A. et al. (2017) Structure. 25:1907.
  5. Mihara, E. et al. (2016) Elife. 5:11621.
  6. Köninger, A. et al. (2018) Arch Gynecol Obstet. 298(5):1009.
  7. Aktas B. et al. (2013). Anticancer Res. 33:329.
  8. Tramontana A. et al. (2018) Clin. Chim. Acta. 476:160.
  9. Kollerits, B. et al. (2017) Diabetes Care. 40:1386.
Entrez Gene IDs
173 (Human); 280662 (Mouse); 282708 (Rat)
Alternate Names
Afamin; AFM; ALB2; ALB2alpha-Alb; ALBA; ALBAalpha-albumin; ALF; Alpha-Alb; Alpha-albumin; MGC125338; MGC125339

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