Recombinant Human Afamin His-tag Protein, CF

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Recombinant Human Afamin His-tag Protein SDS-PAGE
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Recombinant Human Afamin His-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to bind Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) in a functional ELISA. The ED50 for this effect is 0.8-6.4 µg/mL.
Human embryonic kidney cell, HEK293-derived human Afamin protein
Leu22-Asn599, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
67 kDa
71-80 kDa

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE Recombinant Human Afamin His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human Afamin (Catalog # 8065-AF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 71 - 80 kDa and 55 - 70 kDa, respectively.

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Background: Afamin

AFM (Afamin; also known as Alpha -Albumin) is a secreted monomeric glycoprotein of the Alb/Albumin family of molecules. It is expressed by hepatocytes (1), CNS endothelial cells (2) and osteoclasts (3), and circulates in the blood at low μg/mL concentrations. AFM is known to bind and transport vitamin E family molecules, playing an important role for transporting at the blood-brain-barrier (2). It also plays a role in maintaining solubility for transporting Wnt proteins (4, 5). AFM also serves as an osteoclast-derived chemoattractant for preosteoblasts, providing a rational for the observation that bone formation often follows bone resorption (3). Mature human AFM is 578 amino acids (aa) in length (aa 22-599). It contains three consecutive albumin domains (aa 36-206, aa 211-403 and aa 404-599) that contain a characteristic 5 or 6 intrachain disulfide bonds. Full-length human AFM shares 66% aa sequence identity with mouse AFM and 67% aa sequence identity with rat AFM. The importance of Afamin in transport of molecules has led to a suggested diagnostic role in various diseases, including pre-eclampsia (6), ovarian cancer (7), and both gestational and type-2 diabetes (8, 9).

  1. Liu, H. et al. (2011) DNA Cell Biol. 30:137.
  2. Kratzer, I. et al. (2009) J Neurochem. 108(3):707.
  3. Kim, B.J. et al. (2012) Bone. 51:431.
  4. Naschberger, A. et al. (2017) Structure. 25:1907.
  5. Mihara, E. et al. (2016) Elife. 5:11621.
  6. Köninger, A. et al. (2018) Arch Gynecol Obstet. 298(5):1009.
  7. Aktas B. et al. (2013). Anticancer Res. 33:329.
  8. Tramontana A. et al. (2018) Clin. Chim. Acta. 476:160.
  9. Kollerits, B. et al. (2017) Diabetes Care. 40:1386.
Entrez Gene IDs
173 (Human); 280662 (Mouse); 282708 (Rat)
Alternate Names
Afamin; AFM; ALB2; ALB2alpha-Alb; ALBA; ALBAalpha-albumin; ALF; Alpha-Alb; Alpha-albumin; MGC125338; MGC125339


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Recombinant Human Afamin His-tag Protein, CF
By Anonymous on 08/17/2021
Application: Stem/Immune cell maintenance or differentiation

Afamin promote lung organoid growth

Recombinant Human Afamin His-tag Protein, CF
By Anonymous on 07/21/2021
Application: Enzymatic activity in vitro

wnt3a and wnt3a+afmain protein were treated to HEK293SFT cells, and lucferase was done to detect if afamin can enhance the wnt3a activity.