Aldehyde Dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that catalyze the oxidation of endogeneously produced and exogeneous aldehydes to their corresponding acids (1). They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and in lipid peroxidation. ALDH3A1 is also known as stomach aldehyde dehydrogenase. It exists as a homodimer, and prefers NADP+ over NAD+ as its co‑factor (2). It preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates (3). The enzyme is highly expressed in stomach and cornea (2, 4). In the cornea, its proposed roles have been to absorb UV‑light, reduce oxidative damage, maintain corneal refractive and transparence properties, and display chaperone-like activity (4, 5). It also has been identified as a lung cancer biomarker (6).
Recombinant Human Aldehyde Dehydrogenase 3-A1/ALDH3A1, CF
R&D Systems | Catalog # 6705-DH
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 (6705-DH)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human Aldehyde Dehydrogenase 3-A1/ALDH3A1 protein
Ser2-His453, with an N-terminal Met and 6-His tag
Ser2-His453, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
51 kDa
SDS-PAGE
51-56 kDa, reducing conditions
Activity
Measured by the ability to catalyze the oxidation of 4-nitrobenzaldehyde.
The specific activity is >6000 pmol/min/μg, as measured under the described conditions.
The specific activity is >6000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation, and Storage
6705-DH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Aldehyde Dehydrogenase 3-A1/ALDH3A1
References
- Marchitti, S. A. et al. (2007) Pharmacol. Rev. 59:125.
- Yin, S. J. et al. (1991) FEBS Lett. 283:85.
- Hsu, L. C. et al. (1992) J. Biol. Chem. 267:3030.
- Estey, T. et al. (2007) Exp. Eye Res. 84:3.
- Estey, T. et al. (2010) PLOS One. 5:e15218.
- Kim, B. et al. (2007) Cancer Res. 67:7431.
Alternate Names
Aldehyde Dehydrogenase 3A1, ALDH3A1, ALDHIII
Gene Symbol
ALDH3A1
UniProt
Additional Aldehyde Dehydrogenase 3-A1/ALDH3A1 Products
Product Documents for Recombinant Human Aldehyde Dehydrogenase 3-A1/ALDH3A1, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Aldehyde Dehydrogenase 3-A1/ALDH3A1, CF
For research use only
Related Research Areas
Citations for Recombinant Human Aldehyde Dehydrogenase 3-A1/ALDH3A1, CF
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Protocols
View specific protocols for Recombinant Human Aldehyde Dehydrogenase 3-A1/ALDH3A1, CF (6705-DH):
Materials
*Adjusted for Substrate Blank
**Using the extinction coefficient 6270 M-1cm-1
***Using the path correction 0.32 cm
Note: the output of many spectrophotometers is in mOD Per Well:
- Assay Buffer: 50 mM Tris, 5 mM DTT, pH 9.0
- Recombinant Human Aldehyde Dehydrogenase 3-A1/ALDH3A1 (rhALDH3A1) (Catalog # 6705-DH)
- Nicotinamide adenine dinucleotide phosphate (NADP+) (Sigma, Catalog # N5755), 50 mM stock in deionized water
- 4-Nitrobenzaldehyde (4-NBA) (Sigma, Catalog # 72800), 200 mM stock in DMSO
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rhALDH3A1 to 4 ng/µL in Assay Buffer.
- Dilute NADP+ to 2 mM in Assay Buffer.
- Dilute 4-NBA to 4 mM in Assay Buffer.
- Form Substrate Mixture by combining equal volumes of 2 mM NADP+ and 4 mM 4-NBA.
- Load 50 µL of the 4 ng/µL rhALDH3A1 into the plate. Include a Substrate Blank containing 50 µL of Assay Buffer.
- Start the reaction by adding 50 µL of Substrate Mixture to the wells.
- Read plate at 340 nm (absorbance) in kinetic mode for 5 minutes.
- Calculate specific activity:
Specific Activity (pmol/min/µg) = | Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol |
| ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Using the extinction coefficient 6270 M-1cm-1
***Using the path correction 0.32 cm
Note: the output of many spectrophotometers is in mOD Per Well:
- rhALDH3A1: 0.2 µg
- NADP+: 0.5 mM
- 4-NBA: 1 mM
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