Recombinant Human alpha-Synuclein Protein, CF

R&D Systems | Catalog # SP-485

R&D Systems
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human alpha-Synuclein Protein (SP-485)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P37840.1

Applications

Bioactivity
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Product Specifications

Source

E. coli-derived human alpha-Synuclein protein
Met1 - Ala140

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

14 kDa

Activity

Recombinant Human alpha -Synuclein is ideal for use as a control substrate for in vitro Ubiquitin conjugation using select Ubiquitin E3 ligases such as CHIP/Stub1.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial Recombinant Human  alpha -Synuclein concentration of 0.5-2.5 μM.

Scientific Data Images for Recombinant Human alpha-Synuclein Protein, CF

Recombinant Human alpha-Synuclein Protein SDS-PAGE

Recombinant Human alpha-Synuclein Protein SDS-PAGE

0.5 μg and 1 μg per lane of Recombinant Human alpha-Synuclein (Catalog # SP-485) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a single band at 19 kDa.

Formulation, Preparation, and Storage

SP-485
Formulation Supplied as a solution in HEPES, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: alpha-Synuclein

alpha -Synuclein is a member of a family of small, soluble proteins that also include beta -, and gamma -Synuclein. alpha -Synuclein is predominantly expressed in neurons of the central nervous system, specifically in the presynaptic region of nerve terminals.  This protein cycles between a free, partially unfolded form and a helical, membrane-bound form.  alpha -Synuclein can aggregate in vivo and in vitro, forming various oligomeric species and fibrillar and amorphous aggregates.  The fibrils and amyloidal forms are major components of Lewy Bodies and have been linked to the pathogenesis of Parkinson's Disease dementia.  alpha -Synuclein aggregates can also be found associated with amyloid plaques in Alzheimer's Disease.

References

  1. Breydo L. et al. (2012) Biochim. Biophys. Acta.  1822: 261.
  2. Chen R.H. et al.  (2013) J. Biol. Chem.  288: 7438.
  3. Li X. et al. (2008)  Acta. Biochim. Biophys. Sin (Shanghai) 40: 406.
  4. Surguchov A. (2008)  Int. Rev. Cell Mol. Biol. 270: 225.
  5. Xia Q. et al. (2008) Front. Biosci. 13: 3850.

Alternate Names

NACP, PARK1, PARK4, SNCA, Synuclein-alpha

Entrez Gene IDs

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Gene Symbol

SNCA

UniProt

P37840.1

Additional alpha-Synuclein Products

Product Documents for Recombinant Human alpha-Synuclein Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human alpha-Synuclein Protein, CF

For research use only

Related Research Areas

Neuronal Lineage Markers

Citations for Recombinant Human alpha-Synuclein Protein, CF

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FAQs for Recombinant Human alpha-Synuclein Protein, CF

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  • Q: Is Recombinant Human alpha-Synuclein Protein (Catalog # SP‑485) supplied as a monomer?

    A: Based on the known properties of the protein and its behavior under standard conditions, SP‑485 is expected to remain monomeric in solution. However, because this has not been experimentally validated as part of our release criteria, we recommend that customers assess the protein and its oligomeric state within their specific assay or application to ensure it meets performance requirements.

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