Leukocyte-derived arginine aminopeptidase (LRAP) and endoplasmic reticulum-aminopeptidase 2 (ERAP2) are the two names given to the protein encoded by the LRAP gene (1, 2). Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors. It belongs to the oxytocinase subfamily of M1 aminopeptidases, which also includes aminopeptidases PILS/ARTS1/ERAP1 and LNPEP/PLAP (1, 3). In addition to antigen presentation, the members of this subfamily are also important in maintenance of pregnancy, memory retention, and blood pressure regulation (3).
Recombinant Human Aminopeptidase LRAP/ERAP2 Protein, CF
R&D Systems | Catalog # 3830-ZN
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Key Product Details
- R&D Systems NS0-derived Recombinant Human Aminopeptidase LRAP/ERAP2 Protein (3830-ZN)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Aminopeptidase LRAP/ERAP2 protein
Ala56-Thr960, with an N-terminal 6-His tag
Ala56-Thr960, with an N-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
105 kDa
SDS-PAGE
122 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC).
The specific activity is >50 pmol/min/µg, as measured under the described conditions.
The specific activity is >50 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
3830-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Aminopeptidase LRAP/ERAP2
References
- Tanioka, T. et al. (2003) J. Biol. Chem. 278:32275.
- Tanioka, T. et al. (2005) FEBS J. 272:916.
- Tsujimoto, M. and A. Hattori (2005) Biochim. Biophys. Acta 1751:9.
Long Name
Leukocyte-derived Arginine Aminopeptidase
Alternate Names
ERAP2
Entrez Gene IDs
64167 (Human)
Gene Symbol
ERAP2
UniProt
Additional Aminopeptidase LRAP/ERAP2 Products
Product Documents for Recombinant Human Aminopeptidase LRAP/ERAP2 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Aminopeptidase LRAP/ERAP2 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Aminopeptidase LRAP/ERAP2 Protein, CF
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Protocols
View specific protocols for Recombinant Human Aminopeptidase LRAP/ERAP2 Protein, CF (3830-ZN):
Materials
- Assay Buffer: 100 mM MES, pH 6.5
- Recombinant Human Aminopeptidase LRAP/ERAP2 (Catalog # 3830-ZN)
- Substrate: H-Arg-AMC (Bachem, Catalog # I-1050)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhLRAP to 5 µg/mL in Assay Buffer.
- Dilute Substrate to 100 µM in Assay Buffer.
- Load 50 µL of 5 µg/mL rhLRAP into a plate, and start the reaction by adding 50 µL of 100 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate.
- Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 7-Amino-4-Methyl Coumarin (Sigma-Aldrich, Catalog # A9891). Per Well:
- rhLRAP: 0.25 µg
- Substrate: 50 µM
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