The human XPNPEP2 gene encodes Aminopeptidase P2 (APP2), which is also known as X-prolyl Aminopeptidase 2 or membrane bound Aminopeptidase P (1‑4). It is a member of the M24 family of metalloproteases, which also contains methionine Aminopeptidases, X-Pro dipeptidase, Aminopeptidase P1, Aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (5). Mammalian APP2 are predicted to be GPI-anchored membrane proteases and their biological functions have been reviewed (6). Human APP2 is widely expressed in many adult tissues with the highest levels in the kidney (7). The purified recombinant human APP2 corresponds to the ectodomain and is shown here to be an active aminopeptidase, removing a N-terminal amino acid from a peptide that contains a Pro residue at the second position.
Recombinant Human Aminopeptidase P2/XPNPEP2 Protein, CF
R&D Systems | Catalog # 2490-ZN
Key Product Details
- R&D Systems NS0-derived Recombinant Human Aminopeptidase P2/XPNPEP2 Protein (2490-ZN)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
His22-Ala650, with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The specific activity is >300 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
2490-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Aminopeptidase P2/XPNPEP2
References
- Venema, R.C. et al. (1997) Biochim. Biophys. Acta 1354:45.
- Sprinkle, T.J. et al. (1998) Genomics 50:114.
- Cottrell, G.S. et al. (1998) Biochem. Soc. Trans. 26:S248.
- Prueitt, R.L. et al. (2000) Cytogenet. Cell Genet. 89:44.
- Barrett, A.J. et al. (2004) Handbook of Proteolytic Enzymes, Elsevier Academic Press, San Diego.
- Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.) p. 934, Elsevier Academic Press, San Diego.
- Ersahin C. et al. (2005) Arch. Biochem. Biophys. 435:303.
Alternate Names
Gene Symbol
UniProt
Additional Aminopeptidase P2/XPNPEP2 Products
Product Documents for Recombinant Human Aminopeptidase P2/XPNPEP2 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Aminopeptidase P2/XPNPEP2 Protein, CF
The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for non-commercial research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This Product is manufactured under a license to U.S. Patent Nos. 6,399,349 and 7,273,718.
Any party desiring rights under this patent should contact Ryogen LLC, Montebello Park, 75 Montebello Road, Suffern, NY 10901.
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Human Aminopeptidase P2/XPNPEP2 Protein, CF (2490-ZN):
- Assay Buffer: 100 mM HEPES, 0.5 mM MnCl2, pH 8.0
- Recombinant Human Aminopeptidase P2/XPNPEP2 (rhXPNPEP2) (Catalog # 2490-ZN)
- Substrate: Lys(Abz)-Pro-Pro-pNA (Bachem, Catalog # L-1980), 10 mM stock in deionized water
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhXPNPEP2 to 1 ng/µL in Assay Buffer. Incubate at room temperature for 30 minutes.
- Dilute Substrate to 100 μM in Assay Buffer.
- Load into plate 50 μL of 1 ng/µL of rhXPNPEP2 and start the reaction by adding 50 µL of 100 µM Substrate. Include a Substrate Blank of 50 μL Assay Buffer and 50 µL of 100 µM Substrate.
- Read at excitation and emission wavelengths of 320 nm and 410 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard ABZ-Gly (Bachem, Catalog # E-2920).
- rhXPNPEP2: 0.05 µg
- Substrate: 50 µM