Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

R&D Systems | Catalog # 9795-AN

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Angiopoietin-like Protein 2/ANGPTL2 (9795-AN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q9UKU9.1

Applications

Bioactivity
View all Angiopoietin-like Protein 2/ANGPTL2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 2/ANGPTL2 protein
Thr260-His493, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr260

Predicted Molecular Mass

28 kDa

SDS-PAGE

28-36 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Angiopoietin‑like Protein 2/ANGPTL2 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) that produces 50% of the optimal binding response is 6-48 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 9795-AN in the following applications:

Scientific Data Images for Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

Recombinant Human Angiopoietin-like Protein 2/ANGPTL2 Binding Activity

Recombinant Human Angiopoietin-like Protein 2/ANGPTL2 Binding Activity

When Recombinant Human Angiopoietin-like Protein 2/ANGPTL2 (Catalog # 9795-AN) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 6-48 ng/mL.

Formulation, Preparation, and Storage

9795-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 2/ANGPTL2

Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2 is a secreted 56 kDa glycoprotein that contains a N-terminal coiled coil domain and a C-terminal fibrinogen like domain (1). ANGPTL2 is one of the seven members of the Angiopoietin Like family proteins, which are structurally similar to angiopoietins (2). Within mature human ANGPTL2, amino acid 260-493 contains the C-terminal fibrinogen like domain, and this domain shares 99% sequence identity with both mouse and rat homologs. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6-7). ANGPTL2 also function as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled coil domain (8). Receptor of ANGPTL2 is recently identified as LILRB2, and both the coiled coil domain and fibrinogen like domain are required for optimal binding (9).

References

  1. Kim, I. et al. (1999) J Biol Chem 274:26523.
  2. Santulli, G. et al. (2014) Front Endocrinol 5:4.
  3. Tabata, M. et al. (2009) Cell Metab 10:178.
  4. Kubota, Y. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:13502.
  5. Kikuchi, R. et al. (2008) Cancer Res 68:5067.
  6. Odagiri, H. et al. (2014) Sci Signal 7:ra7.
  7. Endo, M. et al. (2012) Cancer Res 72:1784.
  8. Broxmeyer, H.E. et al. (2012) Blood Cells Mol Dis 48:25.
  9. Deng, M. et al. (2014) blood 124:924.

Alternate Names

ANGPTL2, ANGRP2

Entrez Gene IDs

23452 (Human); 26360 (Mouse)

Gene Symbol

ANGPTL2

UniProt

Q9UKU9.1

Additional Angiopoietin-like Protein 2/ANGPTL2 Products

Product Documents for Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

For research use only

Citations for Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

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    Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF 9795-AN

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail